Barnstead easypure water filter system

From Sigma-Aldrich (St. Louis, MO, USA): NaHPO₄·7H₂O, NaH₂PO₄·2H₂O, NaOH, 6-aminocaproic acid, L-Arginine, Nitroblue tetrazolium, Ethilenediaminetetraacetic acid (EDTA), glyoxal, Tween 20, fluorescamine, NaOH, HCl, trichloroacetic acid, glycine, thiobarbituric acid and aminoguanidine hydrochloride. 9,10-Phenanthrenequinone was purchased from Merck (Darmstadt, Germany). Ultrapure water was obtained from a Barnstead EasyPure water filter system (Thermo Scientific, Dubuque, IA, USA) and it was used for the preparation of all solutions.

2,2′-Azobis(2-methyl-propionamidine) dihydrochloride (AAPH), copper sulphate, Chelex® 100 sodium, quercetin, thiobarbituric acid, trichloroacetic acid, Amberlite XAD-7, sodium bicarbonate, methylglyoxal, glutathione (reduced form), 1-chloro-2,4-dinitrobenzene (CDNB), L-glutamine, and CelLytic™ M were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pierce™ BCA Protein Assay Kit was obtained from Thermo Scientific (Rockford, IL, USA). Hydrogen peroxide (30%), HCl, methanol, and formic acid were purchased from Merck (Darmstadt, Germany). MTT was obtained from Calbiochem (Darmstadt, Germany). Fetal bovine serum (FBS), antibiotics, and culture media were purchased from Invitrogen (Grand Island, New York, USA). Ultrapure water was obtained using a Barnstead EasyPure water filter system (Thermo Scientific, Dubuque, IA, USA).