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Bj fibroblasts

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BJ fibroblasts are a cell line derived from human foreskin tissue. They are a type of primary cell line commonly used in cell biology research. BJ fibroblasts can be used for various in vitro experiments and assays.

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Lab products found in correlation

Mia paca 2, by Thermo Fisher Scientific (2 mentions) Glutamax supplement, by Thermo Fisher Scientific (2 mentions) Horse serum, by Thermo Fisher Scientific (2 mentions)

2 protocols using bj fibroblasts

1

Cell Line Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
All cell lines were obtained from the American Type Culture Collection (ATCC) and grown at 37°C, 5% CO2. MIA PaCa-2 (CRL-1420) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose and GlutaMAX™ supplement (Gibco), 10% fetal bovine serum (FBS; HyClone) and 2.5% horse serum (Gibco). BJ fibroblasts (CRL-2522) were cultured in minimal essential medium (MEM) with GlutaMAX™ supplement (Gibco) and 10% FBS (HyClone). SJCRH30 (CRL-2061) cells were cultured in RPMI with GlutaMAX™ supplement (Gibco) and 10% FBS (HyClone).
Vostrosablin N., Lim S., Gopal P., Brazdilova K., Parajuli S., Wei X., Gromek A., Prihoda D., Spale M., Muzdalo A., Greig J., Yeo C., Wardyn J., Mejzlik P., Henry B., Partridge A.W, & Bitton D.A. (2024). mRNAid, an open-source platform for therapeutic mRNA design and optimization strategies. NAR Genomics and Bioinformatics, 6(1), lqae028.
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2

Cell Line Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
All cell lines were obtained from the American Type Culture Collection (ATCC) and grown at 37°C, 5% CO2. MIA PaCa-2 (CRL-1420) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose and GlutaMAX™ supplement (Gibco), 10% fetal bovine serum (FBS; HyClone) and 2.5% horse serum (Gibco). BJ fibroblasts (CRL-2522) were cultured in minimal essential medium (MEM) with GlutaMAX™ supplement (Gibco) and 10% FBS (HyClone). SJCRH30 (CRL-2061) cells were cultured in RPMI with GlutaMAX™ supplement (Gibco) and 10% FBS (HyClone).
Vostrosablin N., Lim S., Gopal P., Brazdilova K., Parajuli S., Wei X., Gromek A., Prihoda D., Spale M., Muzdalo A., Greig J., Yeo C., Wardyn J., Mejzlik P., Henry B., Partridge A.W, & Bitton D.A. (2024). mRNAid, an open-source platform for therapeutic mRNA design and optimization strategies. NAR Genomics and Bioinformatics, 6(1), lqae028.
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