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Dipotassium hydrogen phosphate

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Dipotassium hydrogen phosphate is a chemical compound with the formula K2HPO4. It is a white, crystalline solid that is soluble in water. Dipotassium hydrogen phosphate is commonly used as a buffer in various laboratory applications to maintain a specific pH level.

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Lab products found in correlation

Disodium hydrogen phosphate, by Avantor (2 mentions) Methanol, by Avantor (1 mentions) Absolute methanol, by Merck Group (1 mentions) Orthophosphoric acid, by Avantor (1 mentions) Ammonium reineckate, by Avantor (1 mentions) 4 hydroxybenzaldehyde, by Merck Group (1 mentions) 4 chlorophenol, by Merck Group (1 mentions) Anhydrous toluene, by Merck Group (1 mentions) Potassium dihydrogen orthophosphate, by Avantor (1 mentions) Sodium hydroxide (naoh), by Loba Chemie (1 mentions) Sulfuric acid, by Avantor (1 mentions) Citric acid, by Avantor (1 mentions) Fructose, by Avantor (1 mentions) Sodium chloride (nacl), by Carl Roth (1 mentions) Potassium chloride (kcl), by Carl Roth (1 mentions) Sucrose, by Carl Roth (1 mentions) Peptone, by Carl Roth (1 mentions) Magnesium chloride hexahydrate, by Avantor (1 mentions) Beef extract, by Carl Roth (1 mentions) Sodium hydrogen carbonate, by Avantor (1 mentions)

Spelling variants of this product

Dipotassium phosphate (2 citations) Dipotassium hydrogen phosphate trihydrate (2 citations) Dipotassium hydrogen phosphate (K2HPO4) (2 citations)

7 protocols using dipotassium hydrogen phosphate

1

Formulation and Characterization of AMH

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AMH was purchased from Sigma Aldrich, Hamburg, Germany. Pluronic F-127, Tween 80 and PEG 6000 were purchased from Kosdac, Merk Schuchardt, Germany. Cardarone (200 mg) tablet and heparin were purchased from a Omega pharmacy, Islamabad Pakistan. Concentrated HCl, disodium hydrogen phosphate, dipotassium hydrogen phosphate and methanol were purchased from BDH laboratory supplies, England. Size 0 capsule and avecil-200 was gifted by Wise pharmaceuticals, Pakistan. The rest of the chemicals used were of pure analytical grade.
Awan A.M., Farid A., Shah S.U., Khan D., Ur Rehman F., Dar M.J., Iftikhar T., Ghazanfar S., Galanakis C.M., Alamri A.S., Asdaq S.M, & Shah K.U. (2022). Nanocrytals-Mediated Oral Drug Delivery: Enhanced Bioavailability of Amiodarone. Pharmaceutics, 14(6), 1300.
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2

Photocatalytic Degradation of Organic Pollutants

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TiO2 (anatase), bezafibrate, ferrocenaldehyd, sarcosin and anhydrous toluene were purchased from Sigma-Aldrich. 4-Chlorophenol (CP), 4-chlorobenzoic acid (CBA), 4-hydroxybenzaldehyde (HBD) (all Merck), absolute methanol, dipotassium hydrogen phosphate, orthophosphoric acid (all POCh, Gliwice, Poland) and ammonium reineckate (BDH Chemicals) were used. All above mentioned chemicals were of analytical grade reagents and used without further treatment. HPLC-grade acetonitrile was purchased from J.T. Baker. All solutions were prepared using deionized water, which was obtained by Polwater apparatus.
Regulska E, & Karpińska J. (2014). Investigation of novel material for effective photodegradation of bezafibrate in aqueous samples. Environmental Science and Pollution Research International, 21(7), 5242-5248.
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3

Formulation and Evaluation of Diclofenac Tablets with Brachystegia eurycoma Gum

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Materials. Isopropyl alcohol, acetone, (Guangxing Guanghua Chemical, China), potassium dihydrogen orthophosphate, dipotassium hydrogen phosphate (BDH Chemicals Ltd Poole England), diclofenac, (Alpha Lab, Germany), hydroxylpropyl methylcellulose, lactose, polyvinyl pyrrolidone, magnesium stearate (Mira Organics and Chemical Co. Ltd. Chennai, India), sodium hydroxide (Loba Chemie, Mumbia, India), were of analytical grade. Brachystegia eurycoma gum was isolated from Brachystegia eurycoma seeds obtained from a local market in Abraka, Delta state, Nigeria.
Eziuzo O.S., Avbunudiogba J.A., & Ejomafuvwe E. (2020). Formulation and evaluation of sustained release diclofenac sodium matrix tablets produced using <i>Brachystegia eurycoma </i>gum. Journal of Pharmacy & Bioresources, 17(1), 34-43.
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4

Fructose-Based Analytical Protocol

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Fructose, citric acid, dipotassium hydrogen phosphate and sulfuric acid (98 %, HPLC grade) were purchased from VWR and used as provided. HMF was purchased from Alibaba and purified by vacuum distillation prior to use. Water was deionised. For the HPLC eluent, Millipore grade water was used. The solvents used for LC‐MS (water, methanol, formic acid) were of the respective grade.
Körner P., Jung D, & Kruse A. (2019). Influence of the pH Value on the Hydrothermal Degradation of Fructose. ChemistryOpen, 8(8), 1109-1120.
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5

Antibacterial Cellulose Paper Testing

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Norfloxacin, 95%, was purchased from abcr GmbH (Karlsruhe, Germany). Commercially available, cellulose-based paper (Soft & Sicher, dm-drogerie markt GmbH + Co. KG, Karlsruhe, Germany) was utilised as the paper matrix. Sucrose, peptone, beef extract, sodium chloride and potassium chloride were acquired from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). Agar was purchased from Sigma-Aldrich Pty Ltd. (Darmstadt, Germany). Magnesium sulfate heptahydrate, magnesium chloride hexahydrate, calcium chloride dihydrate, sodium hydrogen carbonate, di-potassium hydrogen phosphate and di-sodium hydrogen phosphate were acquired from VWR International GmbH (Darmstadt, Germany). Aliivibrio fischeri (A. fischeri) bacterial strain (ATCC 7744/ NCMB 1281) was obtained from Dr. G. Schuchardt® (Göttingen, Germany). Purified water was freshly obtained from a PURELAB Flex 2 (ELGA LabWater, Veolia Water Technologies GmbH, Celle, Germany).
Abdelkader A., Nallbati L, & Keck C.M. (2023). Improving the Bioactivity of Norfloxacin with Tablets Made from Paper. Pharmaceutics, 15(2), 375.
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6

Detailed Analytical Reagent Preparation

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The following chemicals were purchased from VWR (Oud-Heverlee, Belgium), dipotassium hydrogen phosphate, potassium dihydrogen phosphate, KCL, and glycerol. Tolbutamide (TB), chlorzoxazone (CZ), chlorpropamide, formic acid, cytochrome c from equine heart, KCN, TEABC, DTT, MMTS, CaCl2, DMSO were purchased from Sigma-Aldrich (St. Louis, MO, United States). Xylazine (Xyl-M® 2%) was obtained from VMD (Arendonk, Belgium). Tiletamine-zolazepam (Zoletil® 100) was purchased from Virbac (Carros, France). Sodium pentobarbital 20%® was obtained from Kela (Hoogstraten, Belgium). The Coomassie Bradford Protein Assay Kit was purchased from Thermo Fisher Scientific (Asse, Belgium). Midazolam (MDZ), 1-hydroxy-midazolam (1-OH-MDZ), 6-hydroxy-chlorzoxazone (6-OH-CZ), and 4-hydroxy-Tolbutamide (4-OH-TB) were all obtained from Alsachim (Illkirch-Graffenstaden, France). NADPH was purchased from Gentaur (Kampenhout, Belgium). UPLC-water and ACN were obtained from Biosolve (Valkenswaard, Netherlands). Trypsin was purchased from Promega (Madison, WI, United States). Beta-galactosidase was purchased from Sciex (Framingham, MA, United States). Hi3 E. coli was purchased from Waters (Zellik, Belgium). The stock solutions of the probe substrates, the metabolites and the internal standard were prepared separately in methanol (MeOH) at a concentration of 1 mg/mL and stored at -80°C.
Millecam J., De Clerck L., Govaert E., Devreese M., Gasthuys E., Schelstraete W., Deforce D., De Bock L., Van Bocxlaer J., Sys S, & Croubels S. (2018). The Ontogeny of Cytochrome P450 Enzyme Activity and Protein Abundance in Conventional Pigs in Support of Preclinical Pediatric Drug Research. Frontiers in Pharmacology, 9, 470.
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7

NAD(P)H Oxidase Assay from Streptococcus

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A water forming NAD(P)H oxidase (NOX) from Streptococcus mutans 29 was kindly supplied by InnoSyn (Geleen, The Netherlands) as a cell free extract (CFE) formulation. Potassium dihydrogen phosphate and dipotassium hydrogen phosphate, purchased from VWR (Radnor, PA, USA), were used to prepare potassium phosphate buffer. NADPH tetrasodium salt (β-nicotinamide-adenine dinucleotide phosphate, reduced) was acquired from VWR (Radnor, PA, USA).
NOX activity was on average 850 U/mLCFE. One unit corresponds to 1 µmol of NADPH consumed per minute.
Dias Gomes M., Moiseyenko R.P., Baum A., Jørgensen T.M, & Woodley J.M. (2019). Use of image analysis to understand enzyme stability in an aerated stirred reactor. Biotechnology progress, 35(6).
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