The mature fruits of P. foetida were harvested from Dongfang city, Hainan province, China, in May 2021. RAW264.7 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Dulbecco’s Modified Eagle’s medium (DMEM) was obtained from Gibco BRL (Gaithersburg, MD, USA). Penicillin, streptomycin, and fetal bovine serum (FBS) were products of HyClone (Logan, UT, USA). Lipopolysaccharide (LPS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), sulfuric acid, trifluoroacetic acid (TFA), enhanced chemiluminescence (ECL) kit, AB-8 macroporous resin, and polyamide filler were produced by Yuanye Biotechnology Co., Ltd. (Shanghai, China). Bicinchoninic acid (BCA) kit, nitric oxide (NO) detection kit, ELISA kits (TNF-α and IL-6), and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Sephadex LH-20 dextran gel was purchased from General Electric Co. (GE, Fairfield, CT, USA). The methanol and acetonitrile were produced by Sigma Chemical Co. (St. Louis, MO, USA).
Ab 8 macroporous resin
Manufactured by Yuanye Bio-Technology
Sourced in China
The AB-8 macroporous resin is a type of chromatographic media used for the purification and separation of various compounds. It features a large surface area, high adsorption capacity, and excellent mechanical properties. The AB-8 resin is designed to facilitate efficient and reliable chromatographic processes, though its specific applications and intended use are not provided.
Automatically generated - may contain errors
Lab products found in correlation
Sulfuric acid, by Yuanye Bio-Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Nitric oxide detection kit, by Beyotime (1 mentions)
Trifluoroacetic acid tfa, by Yuanye Bio-Technology (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Raw264.7 cell, by American Type Culture Collection (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Bicinchoninic acid bca kit, by Beyotime (1 mentions)
Ho 1 antibody, by Proteintech (1 mentions)
Keap1 antibody, by Proteintech (1 mentions)
Nrf2 antibody, by Proteintech (1 mentions)
Tlr4 antibody, by Proteintech (1 mentions)
Gapdh antibody, by Proteintech (1 mentions)
Hplc grade acetonitrile acn, by Thermo Fisher Scientific (1 mentions)
Deae cellulose, by Yuanye Bio-Technology (1 mentions)
3 protocols using ab 8 macroporous resin
1
Bioactive Compounds from P. foetida Fruits
2
Hepatoprotective Effects of HTF
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The HTF was obtained by the method of Cheng et al. with some modifications. 95% ethanol (analytical-grade) was purchased from the Chengdu Chron Chemicals Co., Ltd. (Chengdu, Sichuan, China). The AB-8 macroporous resin was purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Cytokines in serum and liver (interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α)) were measured using commercial kits purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Both serum and liver biochemical indicators (high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), triglyceride (TG), total cholesterol (TC), glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT)) were measured using Nanjing Jiancheng commercial kits. Antibodies in the experiment were purchased from Proteintech Group, Inc. (Wuhan, Hubei, China), including the Nrf2 antibody, HO-1 antibody, TLR4 antibody, MyD88 antibody, GAPDH antibody, and Keap1 antibody.
3
Cimicifuga heracleifolia Rhizome Analysis
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Dried Cimicifuga heracleifolia Kom. rhizomes (planted in Fushun, Liaoning China) were purchased in the herbal medicine market. AB-8 macroporous resin and DEAE-Cellulose were purchased from Yuanye Bio-Technology Company Limited (Shanghai, China). Bio-gel P-4 extra fine was purchased from Bio-Rad company (Hercules, CA, USA). HPLC grade acetonitrile (ACN) and 50% (w/w) sodium hydroxide (NaOH) solution for HPAEC were purchased from ThermoFisher Scientific (Waltham, MA, USA). Anhydrous sodium acetate (NaAc) for HPAEC study was obtained from J&K Scientific Ltd (Beijing, China). Saccharide standards, including mannose (Man), glucose (Glc), galactose (Gal), fructose (Fru), rhamnose (Rha), fucose (Fuc), xylose (Xyl), arabinose (Ara), galacturonic acid (GalA), glucuronic acid (GlcA), sucrose (Suc) and dextran with different molecular weights (i.e. Mw 1 kDa, 5 kDa, 25 kDa, 50 kDa, 80 kDa), were obtained from Sigma (St. Louis, MO, USA). Inulin standard (extracted from dahlia tubers) was purchased from Aladdin Biochemical Technology Co., Ltd (Shanghai, China). All other chemicals and reagents were of analytical grade made in China.
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