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Cd45 2 percp cy5

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CD45.2-Percp-Cy5.5 is a fluorescently-labeled antibody that binds to the CD45.2 antigen. It can be used for the detection and analysis of cells expressing CD45.2 by flow cytometry.

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Lab products found in correlation

Foxp3 pe, by Thermo Fisher Scientific (2 mentions) Il 10 pe, by Thermo Fisher Scientific (2 mentions) Ifn γ pe cy7, by BD (2 mentions) Cd4 pe, by Thermo Fisher Scientific (2 mentions) Cd8 alexa 647, by BD (2 mentions) Sodium orthovandate na3vo4, by Merck Group (2 mentions) Cd45.2 fitc, by Thermo Fisher Scientific (2 mentions) Cd4 fitc, by Thermo Fisher Scientific (2 mentions) 12 o tetradecanoyl phorbol 13 acetate tpa, by LC Laboratories (2 mentions) Cd8 pe, by BD (2 mentions) Dnase 1 dn25, by Merck Group (1 mentions) Facscanto system, by BD (1 mentions) Cd11c pe cy7, by Thermo Fisher Scientific (1 mentions) Cd326 pe, by Thermo Fisher Scientific (1 mentions) Fc block, by BD (1 mentions) Il 23r, by R&D Systems (1 mentions) 2 o dibutyryladenosine 3 5 cyclic monophosphate dbcamp, by Merck Group (1 mentions) Il 17 percp cy5, by Thermo Fisher Scientific (1 mentions) Cd4 pe cy7, by Thermo Fisher Scientific (1 mentions) Ly6c pecy7, by Thermo Fisher Scientific (1 mentions)

5 protocols using cd45 2 percp cy5

1

Flow Cytometry Antibody Protocol

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Antibodies for use in flow cytometry were obtained from eBioscience (CD45.2-PerCPCy5.5, CD16/CD32, CD3e-PEeFl.610, CD4-APCeFl.780, CD335-APCeFl.780, CD122-PECy7, IFN-γ-PerCPCy5.5, and F4/80-APCeFl.780) and Leinco (CD8-FITC and Ly6g-APC). Depleting antibodies were obtained from Leinco (GK1.5 for anti-CD4-depleting antibody and YTS-169 for anti-CD8-depleting antibody) and graciously donated by Dr. Helen Mullen (University of Missouri) (YTS-156 anti-CD8-depleting antibody). Collagenase/dispase Version 16 was purchased from Roche and resuspended according to manufacturer’s directions. DNaseI DN-25 was purchased from Sigma and stored at −20°C in 2 mg/mL 50% glycerol and 75 mM NaCl.
Lasky C.E., Pratt C.L., Hilliard K.A., Jones J.L, & Brown C.R. (2016). T Cells Exacerbate Lyme Borreliosis in TLR2-Deficient Mice. Frontiers in Immunology, 7, 468.
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2

Multicolor Flow Cytometry Profiling

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Dissociated lung cells were treated with Fc block (BD Biosciences) before staining with the following fluorophore-conjugated antibodies obtained from eBioscience: CD326-PE, CD45.2-PerCP-Cy5.5, CD11c-PE-Cy7, and CD31-APC. Viability was assessed with LIVE/DEAD fixable near-IR cell stain (Life Technologies). No fluorescein isothiocyanate (FITC)-conjugated antibody was employed to help identify autofluorescent alveolar macrophages. Flow cytometry analysis was performed on a BD FACSCanto system.
Ng B., Cash-Mason T., Wang Y., Seitzer J., Burchard J., Brown D., Dudkin V., Davide J., Jadhav V., Sepp-Lorenzino L, & Cejas P.J. (2019). Intratracheal Administration of siRNA Triggers mRNA Silencing in the Lung to Modulate T Cell Immune Response and Lung Inflammation. Molecular Therapy. Nucleic Acids, 16, 194-205.
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3

Immune Response Modulation Protocol

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7,12-dimethylbenz(a)anthracene (DMBA) (≥ 95% purity), N6, 2’-O-dibutyryladenosine 3:5-cyclic monophosphate (dbcAMP) and Sodium orthovandate (Na3VO4) were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO). 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was obtained from LC laboratories (Woburn, MA). Rat anti-mouse IL-12Rβ2 and IL-23R were purchased from R&D; Rabbit anti-mouse VEGF, TRP2, Mouse anti-human S100, Rat anti-mouse vimentin were obtained from Santa Cruz Biotechnology, Inc. Rat anti-mouse pERK was from BD biosciences. CD4-PE, CD4-FITC, FOXP3-PE, FOXP3-v450, IA/IE-FITC, IL-17-Percp-Cy5.5, IL-10-PE, CD45.2-Percp-Cy5.5, CD45.2-FITC were obtained from eBiosciences. IFNγ-PE-Cy7, CD8-Alexa-647, and CD8-PE were obtained from BD-pharmingen.
Nasti T.H., Cochran J.B., Vachhani R.V., McKay K., Tsuruta Y., Athar M., Timares L, & Elmets C.A. (2016). IL-23 Inhibits Melanoma Development by Augmenting DNA Repair and Modulating T-cell Subpopulations. Journal of immunology (Baltimore, Md. : 1950), 198(2), 950-961.
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4

Comprehensive Lung Cell Profiling by Flow Cytometry

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At 4 and 7 dpi, we isolated lung cells as previously described [38 ] and assessed lung cellular composition. Cells were stained at 4°C for 20 min with fluorescently labeled antibodies against the following cell surface markers purchased from eBiosciences unless otherwise noted: Siglec F PE (clone IRNM44N), CD11b APC (clone M1/70), CD11c Efluor 450 (clone N418), Ly6C PE-Cy7 (clone HK1.4), Ly6G PerCP-Cy5.5 (Tonbo, clone 1A8), CD45.2 FITC (clone 104) [Stain 1]; TCRαβ FITC (clone H57-597), TCRγδ APC (clone eBioGL3), CD4 PE-Cy7 (clone GK1.5), CD8 Efluor 450 (clone 53–6.7), DX5 PE (clone DX5), CD45.2 PerCP-Cy5.5 (clone 104) [Stain 2]. The cells were fixed with 1% formalin and analyzed on the LSR II flow cytometer (BD Biosciences). CD45.2-gated hematopoietic-derived leukocyte populations were characterized as follows: inflammatory monocytes (IM; Siglec F− CD11bhigh CD11clow Ly6C+), neutrophils (Siglec F− CD11bhigh CD11clow Ly6G+), alveolar macrophages (AM; Siglec Fhigh CD11bint), eosinophils (Siglec Fhigh CD11bhigh), NK cells (αβ TCR− DX5+), CD4 T cells (αβ TCR+ CD4+), CD8 T cells (αβ TCR+ CD8+), and γδ T cells (αβ TCR− γδ TCR+).
Latha K., Patel Y., Rao S, & Watford W.T. (2022). The Influenza-Induced Pulmonary Inflammatory Exudate in Susceptible Tpl2-Deficient Mice Is Dictated by Type I IFN Signaling. Inflammation, 46(1), 322-341.
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5

DMBA-Induced Immune Cell Analysis

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7,12-dimethylbenz(a)anthracene (DMBA) (≥95% purity); 1-fluoro-2,4-dinitrobenzene (DNFB); N6, 2’-O-dibutyryladenosine 3:5-cyclic monophosphate (dbcAMP); and sodium orthovandate (Na3VO4) were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO). 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was obtained from LC laboratories (Woburn, MA). CD4-PE (RRID:AB_465506), CD4-FITC (AB_464892), FOXP3-PE (AB_465936), IL-10-PE (AB_466176), CD45.2-Percp-Cy5.5 (AB_953590), and CD45.2-FITC (AB_465061) were obtained from eBiosciences. FOXP3-v450 (AB_10611728), IFNγ-PE-Cy7 (AB_396766), CD8-Alexa-647 (AB_396792), and CD8-PE (AB_394571) were obtained from BD-Pharmingen.
Nasti T.H., Yusuf N., Sherwani M.A., Athar M., Timares L, & Elmets C.A. (2020). Regulatory T cells play an important role in the prevention of murine melanocytic nevi and melanomas. Cancer prevention research (Philadelphia, Pa.), 14(2), 165-174.
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