1,2-Dioleoyl-sn-glycero-3-phosphatidyl ethanolamine (DOPE) was purchased from the NOF Corporation (Tokyo, Japan). Sphingomyelin (SM) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). STR-S2 (stearylated-Dmt-d -Arg-FK-Dmt-d -Arg-FK-NH2, Dmt = 2,6-dimethyltyrosne) was obtained from the Toray Research Center (Tokyo, Japan). The synthesis of methylated β-cyclodextrin-threaded acid-labile PRX (Me-PRX) and BODIPY-labeled Me-PRX [14 (link)] are described in the Supplementary Materials . HeLa human cervix carcinoma cells were obtained from the RIKEN Cell Bank (Tsukuba, Japan). Dulbecco’s modified Eagle’s medium (low glucose) (DMEM) was obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Fetal bovine serum (FBS) was obtained from Sigma–Aldrich (St. Louis, MO, USA). CellLight ER-RFP BacMam 2.0, LysoTracker Red DND-99 and MitoTracker Deep Red FM were obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals used were commercially available reagent-grade products.
Hela human cervix carcinoma cells
Manufactured by RIKEN Cell Bank
Sourced in Japan
HeLa human cervix carcinoma cells are a well-established immortalized cell line derived from cervical cancer cells. They are widely used in biomedical research as a model system for studying various cellular processes and disease mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Celllight er rfp bacmam 2, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Mitotracker deep red fm, by Thermo Fisher Scientific (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
Sphingomyelin sm, by Avanti Polar Lipids (1 mentions)
Chloroquine, by Fujifilm (1 mentions)
Luciferase assay reagent, by Promega (1 mentions)
Endofree plasmid mega kit, by Qiagen (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Reporter lysis buffer, by Promega (1 mentions)
2 protocols using hela human cervix carcinoma cells
1
Lipid-based Delivery System Synthesis and Characterization
2
HeLa Cell Luciferase Assay Protocol
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HeLa human cervix carcinoma cells were obtained from the RIKEN Cell Bank (Japan). Chloroquine was purchased from WAKO Pure Chemicals (Osaka, Japan). The reporter plasmid pcDNA3.1(+)-luc (7037 bp) encoding the firefly luciferase gene was purified using a Qiagen Endofree Plasmid Mega Kit (Qiagen GmbH, Helden, Germany). Dulbecco`s modified eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from Invitrogen Corp.
(Carlsbad, CA, USA). The luciferase assay reagent and reporter lysis buffer were obtained from Promega Co. (Madison, USA). All other reagents were reagent-grade and commercially available.
(Carlsbad, CA, USA). The luciferase assay reagent and reporter lysis buffer were obtained from Promega Co. (Madison, USA). All other reagents were reagent-grade and commercially available.
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