High sensitivity

Manufactured by Agilent Technologies

The High Sensitivity lab equipment product from Agilent Technologies is designed to provide accurate and precise measurements with exceptional sensitivity. It is a versatile instrument suitable for a wide range of applications in analytical laboratories.

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Lab products found in correlation

Nebnext artic sars cov 2 fs kit, by New England Biolabs (2 mentions) Qubit br dsdna kit, by Agilent Technologies (2 mentions) Miseq version 2 300 cycle kit, by Illumina (2 mentions) Miseq instrument, by Illumina (2 mentions) Viral rna xpress kit, by Omega Bio-Tek (2 mentions) Tapestation 2200, by Agilent Technologies (2 mentions) Lunascript rt supermix kit, by New England Biolabs (2 mentions) Rna tapestation, by Agilent Technologies (1 mentions) Smrtbell express template prep kit 2, by Pacific Biosciences (1 mentions) Sequel 2 system, by Pacific Biosciences (1 mentions) Direct zol miniprep kit, by Zymo Research (1 mentions) Qubit dsdna high sensitivity kit, by Thermo Fisher Scientific (1 mentions) Tapestation 4200, by Agilent Technologies (1 mentions) Qubit rna high sensitivity kit, by Thermo Fisher Scientific (1 mentions) Nextseq 500, by Illumina (1 mentions) Rneasy micro kit, by Qiagen (1 mentions)

4 protocols using high sensitivity

Rapid SARS-CoV-2 Genome Sequencing Workflow

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Total RNA was isolated from VTM within 72 hours of specimen collection using the Omega Bio-Tek Viral RNA Xpress kit (Norcross, GA) on an automated extraction platform. Presence of SARS-CoV-2 RNA was determined by using CDC primers and probes with LunaScript RT Supermix Kit (NEB) run on BioRad (Hercules, CA) C1000 real-time PCR machines. cDNA, PCR amplification, and library preparation was performed using the NEBNext ARTIC SARS-CoV-2 FS kit (NEB) according to the manufacturer’s instructions. Libraries were quantified using Invitrogen Qubit BR dsDNA kit and size determined on an Agilent Tapestation 2200 using high sensitivity d1000 tapes (Santa Clara, CA). Libraries were loaded at 10.5 pM on an Illumina (San Diego, CA) MiSeq version 2, 300 cycle kit on an Illumina MiSeq instrument. Consensus sequences were generated with an in-house data analysis pipeline using bwa, samtools, and ivar.

Kim P.Y., Kim A.Y., Newman J.J., Cella E., Bishop T.C., Huwe P.J., Uchakina O.N., McKallip R.J., Mack V.L., Hill M.P., Ogungbe I.V., Adeyinka O., Jones S., Ware G., Carroll J., Sawyer J.F., Densmore K.H., Foster M., Valmond L., Thomas J., Azarian T., Queen K, & Kamil J.P. (2022). A collaborative approach to improve representation in viral genomic surveillance. bioRxiv.

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Ribo-STAMP and APOBEC1 cell lines

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Plasmid construction, cell culture conditions and maintenance, and generation of doxycycline (dox)-inducible HEK293XT Ribo-STAMP (RPS2-APOBEC1) and APOBEC1-only stable cell lines were completed in accordance with methods outlined by Brannan et al. (2021) (link). For stable cell Ribo-STAMP and APOBEC1-only protein expression, cells were induced with 1ug/mL dox for 72h. Total RNA was isolated from technical triplicate samples of HEK293XT cells expressing Ribo-STAMP and APOBEC1-only constructs using TRIzol extraction and column purification using the Direct-zol Miniprep kit (Zymo Research). Poly(A) selection was completed using the Poly(A) mRNA Magnetic Isolation Module (NEB E7490L) and RNA quality was assessed using high-sensitivity RNA Tapestation (Agilent, . Long-read RNA-seq libraries were prepared using the PacBio Iso-Seq Express protocol (101-763-800) and PacBio SMRTbell Express Template Prep Kit 2.0 (100-938-900). Samples were barcoded using the PacBio Barcoded Overhang Adapter Kit (101-791-700) and then pooled in an equimolar fashion. Samples were sequenced on a SMRT cell 8M with a 30-hour movie time on the PacBio Sequel II system.

Jagannatha P., Tankka A.T., Lorenz D.A., Yu T., Yee B.A., Brannan K.W., Zhou C.J., Underwood J.G, & Yeo G.W. (2024). Long-read Ribo-STAMP simultaneously measures transcription and translation with isoform resolution. Genome research.

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Rapid SARS-CoV-2 Genome Sequencing Workflow

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Kim P.Y., Kim A.Y., Newman J.J., Cella E., Bishop T.C., Huwe P.J., Uchakina O.N., McKallip R.J., Mack V.L., Hill M.P., Ogungbe I.V., Adeyinka O., Jones S., Ware G., Carroll J., Sawyer J.F., Densmore K.H., Foster M., Valmond L., Thomas J., Azarian T., Queen K, & Kamil J.P. (2023). A collaborative approach to improving representation in viral genomic surveillance. PLOS Global Public Health, 3(7), e0001935.

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Sequencing of Memory CD4+ T Cells

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RNA was extracted from FACS sorted target memory CD4+ T cells with RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. For preparation of RNA-Sequencing libraries, RNA concentration was measured using the Qubit RNA High Sensitivity kit (Life Technologies) and quality checked on the 4200 Tapestation using either the High Sensitivity or standard RNA ScreenTape assay (Agilent Technologies), depending on the measured RNA concentrations. PolyA-tailed mRNA was separated for sequencing during library preparation. Libraries were prepared using KAPA's mRNA HyperPrep kit (Roche Diagnostics) according to the manufacturer's instructions using an input of up to 200ng and a fragmentation incubation time of 8 minutes at 94°C. Samples were sequenced on Illumina's NextSeq500 (Illumina Cambridge) using a high output 75 cycle paired-end run. 24 libraries were multiplexed in the same run. Libraries were pooled in equimolar quantities, calculated from concentrations measured using the Qubit dsDNA High Sensitivity kit (Life Technologies) and fragment analysis using the D1000 High Sensitivity assay on the 4200 Tapestation (Agilent Technologies).

Reuschl A., Shivkumar M., Mesner D., Pallett L.J., Guerra‐Assunção J.A., Madansein R., Dullabh K.J., Sigal A., Thornhill J., Li J., Fidler S., Noursadeghi M., Maini M.K., & Jolly C. (2021). HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells.

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