Gryphax naos 20 megapixel full hd usb 3.0 color digital microscope camera

After imaging retinal and choroidal flat-mounts, select areas were excised and postfixed flat in one-quarter strength Karnovsky's paraformaldehyde–glutaraldehyde fixative at 4°C. Tissues were dehydrated and embedded in glycol methacrylate (JB-4; Polysciences, Inc., Warrington, PA, USA) as previously described.¹⁷ (link) Sections (2.5 microns thick) were cut using a dry glass knife on a Sorvall MT2-B Microtome (Norwalk, CT, USA), dried on glass slides, and stained with periodic acid/Schiffs and hematoxylin. Images were captured on a Zeiss Photomicroscope II (Carl Zeiss, Inc.) using a Gryphax NAOS 20 Megapixel Full HD USB 3.0 Color Digital Microscope Camera (Jenoptik).

Whole globes were opened at the limbus, anterior segments were removed, and the posterior eyecups were examined microscopically (Zeiss Stemi 2000-C Stereo Microscope; Carl Zeiss, Inc.). One eye from each donor was cryopreserved while the second eye was processed for retinal and choroidal flat-mounts. Digital images of the eyecups were captured (Gryphax NAOS 20 Megapixel Full HD USB 3.0 Color Digital Microscope Camera; Jenoptik, Rochester Hills, MI, USA) using reflected and transmitted illumination prior to further dissection. Vitreous was removed and retinas were then excised from the RPE/choroid and placed overnight in 2% paraformaldehyde (PFA) in 0.1 M cacodylate buffer at 4°C. Eye cups containing the choroid with the RPE intact were reimaged before they were immersed in 1% EDTA (disodium salt; dihydrate crystal; Baker Chemical Co., Radnor, PA) in distilled water for 2 hours at room temperature to facilitate removal of the RPE. Any adherent RPE cells were removed by pipetting the choroid with EDTA solution from a syringe with a blunted 25-gauge needle. Gross digital images of choroids were captured again without RPE. RPE-denuded choroids were then dissected from the sclera, washed briefly in 0.1 M cacodylate, and fixed overnight in 2% PFA in 0.1 M cacodylate buffer at 4°C.