Conditioned media was collected from normal oral fibroblasts cells (n = 5 donors) maintained in monocellular (normal oral fibroblasts only) 3D cultures at similar passages, and analyzed for levels of various growth factors and cytokines by using the Widescreen Human Cancer Panel 2 (Novagen) with Luminex beads (R&D Systems).
Luminex beads
Manufactured by R&D Systems
Luminex beads are color-coded microspheres used in multiplex assays. They are designed to enable the simultaneous detection and quantification of multiple analytes in a single sample. The beads are coated with specific capture antibodies or ligands that can bind to target molecules, allowing for the measurement of multiple analytes in parallel.
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Lab products found in correlation
2 protocols using luminex beads
1
Profiling Fibroblast Secretome
2
Quantifying Growth Factors in ADSC Cultures
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Basal medium, media collected from ADSC monocultures, media collected from ADSC/ECFC co-cultures, and media collected from cocultures treated with VEGF were analyzed for growth factors using Luminex beads according to the manufacturers' protocol (R&D Systems and Millipore).
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