Mouse monoclonal anti tubulin

ARS and DHA were purchased from Holleypharm (Chongqing, China). FTS was purchased from Santa Cruz Biotechnology (California, USA). DHA, ARS and FTS solutions were prepared by dissolving them in dimethylsulphoxide (DMSO, Sigma, USA) before experiments. The final concentration of DMSO was less than 1% in all experiments. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). 2′, 7′-dichlorodihydrofluorescein diacetate (DCF-DA) and N-acetyl-cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, USA). Mito-Tracker Deep Red 633 was purchased from Invitrogen (Massachusetts, USA). Ac-DEVD-CHO and Z-VAD-FMK were purchased from Beyotime (Jiangsu, China). Z-IETD-FMK was purchased from Selleckchem (USA). Mouse monoclonal anti-Bak antibody (Ab-2) and anti-Bax antibody (6A7) were purchased from Calbiochem (San Diego, CA, USA). Rabbit monoclonal anti-Bid and mouse monoclonal anti-Tubulin, anti-Bax and anti-Bak antibody were purchased from Cell Signaling Technology (Beverly, MA, USA). Proper goat anti-mouse and goat anti-rabbit secondary antibodies were purchased from Invitrogen (Massachusetts, USA). Turbofect^™ transfection regent was purchased from Thermo Fisher Scientific (Massachusetts, USA).