Pierce pes 3k

Manufactured by Thermo Fisher Scientific

The Pierce PES 3K is a centrifugal filter device used for sample concentration and buffer exchange. It features a polyethersulfone (PES) membrane with a 3,000 Dalton molecular weight cutoff.

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Lab products found in correlation

Bio plex mouse cytokine assay, by Bio-Rad (2 mentions)

2 protocols using pierce pes 3k

Cytokine Profiling in Mesothelioma

Cited 1 time

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Peritoneal lavage fluids were concentrated by ultrafiltration through a low‐adsorption polyethersulfonate (PES) membrane (mol. Mass. cutoff 3 kDa, concentrator Pierce PES 3K, Thermo Fisher). The average concentration factor was 4.2 with a range from 3.1–5 and it was used to calculate nonconcentrated levels. A Bio‐Plex mouse cytokine assay (BioRad) for simultaneous quantification of the concentrations of several signaling molecules (Il‐6, G‐Csf/Csf3, M‐Csf/Csf1, Ifn‐γ, Ccl2, Cxcl9, Cxcl10) was run according to the recommended procedure. The concentrations were then normalized to the relative CD45⁺CD68⁺F480⁺ tumor‐associated macrophage population, which represents the major source of these cytokines in the peritoneal environment of mesothelioma [15 (link), 37 (link)].

Hariharan A., Qi W., Rehrauer H., Wu L., Ronner M., Wipplinger M., Kresoja‐Rakic J., Sun S., Oton‐Gonzalez L., Sculco M., Serre‐Beinier V., Meiller C., Blanquart C., Fonteneau J., Vrugt B., Rüschoff J.H., Opitz I., Jean D., de Perrot M, & Felley‐Bosco E. (2022). Heterogeneous RNA editing and influence of ADAR2 on mesothelioma chemoresistance and the tumor microenvironment. Molecular Oncology, 16(22), 3949-3974.

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Cytokine Profiling of Peritoneal Lavage

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Peritoneal lavage fluids were concentrated by ultrafiltration through a low-adsorption polyethersulfonate (PES) membrane (mol. mass. cutoff 3 kDa, concentrator Pierce PES 3K, Thermofisher). The concentration factor was noted for each fluid and used in the calculation of the results. The average concentration factor was 7 with a range from 4 to 10 and it was used to calculate non-concentrated levels. A Bio-Plex mouse cytokine assay (BioRad) for simultaneous quantification of the concentrations of several signaling molecules (IL-6, IL-10, G-CSF, GM-CSF, IFN-γ, CXCl1, CCL2, CCL5, and VEGF) was run according to the recommended procedure.

Rehrauer H., Wu L., Blum W., Pecze L., Henzi T., Serre-Beinier V., Aquino C., Vrugt B., de Perrot M., Schwaller B, & Felley-Bosco E. (2018). How asbestos drives the tissue towards tumors: YAP activation, macrophage and mesothelial precursor recruitment, RNA editing, and somatic mutations. Oncogene, 37(20), 2645-2659.

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