The assay was carried out according to the method mentioned in a previous study [23 (link)]. We collected breast cancer tissues from patients from the First Affiliated Hospital of China Medical University. The ISH probes were ordered from BOSTER Biological Technology Co., Ltd. (USA). For the IHC assays, we collected breast cancer tissues from different groups of subcutaneous. The details of all antibodies used are shown in Table S1 .
Ish probes
Manufactured by Boster Bio
Sourced in United States
ISH probes are molecular biology tools used to detect and localize specific nucleic acid sequences within cells or tissue samples. They are designed to hybridize with target sequences, enabling visualization and analysis of gene expression or the presence of specific DNA or RNA molecules.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ish probes
1
Breast Cancer Tissue Collection and Analysis
2
Evaluating lncRNA FOXD3-AS1 in Implant Tumors
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The expression of lncRNA FOXD3-AS1 of implant tumor in vivo was evaluated with the ISH Kit (Boster Biological Technology; China). The ISH probes targeting lncRNA FOXD3-AS1 were obtained from Boster and ISH assays were performed according to the manufacturer’s procedure.
3
Biotin Pull-Down Assay for lncRNA Analysis
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The assay was carried out according to the method mentioned in a previous study [23] . We collected breast cancer tissues from patients from the First A liated Hospital of China Medical University. The ISH probes were ordered from BOSTER Biological Technology Co., Ltd. (USA). For the IHC assays, we collected breast cancer tissues from different groups of subcutaneous. The details of all antibodies used are shown in Table S1.
Biotin pull-down MCF-7 cell lysates were incubated with CBR3-AS1 or control probe (Sangon, China) conjugated to streptavidin agarose resin beads (Thermo Fisher Scienti c, USA) to generate probe-bound Dynabeads.
After the samples were washed with buffer, DNase I (20 U) was added, and puri ed RNA was collected. The puri ed RNA was analysed by qRT-PCR. The whole experimental process and the buffer formulation were described by Hsu et al [24] .
Biotin pull-down MCF-7 cell lysates were incubated with CBR3-AS1 or control probe (Sangon, China) conjugated to streptavidin agarose resin beads (Thermo Fisher Scienti c, USA) to generate probe-bound Dynabeads.
After the samples were washed with buffer, DNase I (20 U) was added, and puri ed RNA was collected. The puri ed RNA was analysed by qRT-PCR. The whole experimental process and the buffer formulation were described by Hsu et al [24] .
4
Biotin Pull-Down Assay for lncRNA Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The assay was carried out according to the method mentioned in a previous study [23] . We collected breast cancer tissues from patients from the First A liated Hospital of China Medical University. The ISH probes were ordered from BOSTER Biological Technology Co., Ltd. (USA). For the IHC assays, we collected breast cancer tissues from different groups of subcutaneous. The details of all antibodies used are shown in Table S1.
Biotin pull-down MCF-7 cell lysates were incubated with CBR3-AS1 or control probe (Sangon, China) conjugated to streptavidin agarose resin beads (Thermo Fisher Scienti c, USA) to generate probe-bound Dynabeads.
After the samples were washed with buffer, DNase I (20 U) was added, and puri ed RNA was collected. The puri ed RNA was analysed by qRT-PCR. The whole experimental process and the buffer formulation were described by Hsu et al [24] .
Biotin pull-down MCF-7 cell lysates were incubated with CBR3-AS1 or control probe (Sangon, China) conjugated to streptavidin agarose resin beads (Thermo Fisher Scienti c, USA) to generate probe-bound Dynabeads.
After the samples were washed with buffer, DNase I (20 U) was added, and puri ed RNA was collected. The puri ed RNA was analysed by qRT-PCR. The whole experimental process and the buffer formulation were described by Hsu et al [24] .
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