Mice metabolic hormones were determined using either specialized ELISA kit or Milliplex MAP Magnetic Bead Mouse Metabolic Hormone Panel (Millipore MMHMAG-44K, Merck, Germany). Plasma leptin and insulin were determined respectively by mouse leptin ELISA Kit (MultiSciences, China) and mouse insulin ELISA Kit (Beyotime, China) according to manufacturer’s instructions. The Milliplex panel was custom-designed for the simultaneous quantification of GIP (total), insulin, leptin and PYY (total). Plasma samples (10 μL) were assayed using a Luminex MAGPIX analyzer (Merck Millipore, Germany) and the data were processed by Milliplex Analyst 5.1 software and R v3.3.2 (R Core Team, 2016).
Mouse insulin elisa kit
Manufactured by Beyotime
Sourced in China
The Mouse Insulin ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of insulin levels in mouse samples. The kit utilizes a specific antibody coated on a microplate to capture insulin from the sample, and a detection antibody is used to quantify the captured insulin. The assay provides a reliable and sensitive method for the detection and quantification of mouse insulin in various sample types.
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Lab products found in correlation
2 protocols using mouse insulin elisa kit
1
Comprehensive Mouse Metabolic Hormone Analysis
2
Serum Insulin Levels in C57BL/6J Mice
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The serum was collected from C57BL/6J mice and insulin levels were detected by Mouse Insulin ELISA Kit Beyotime Biotech, China) following the manufacturer's instructions. Each sample were performed in triplicate, and the absorbance at 450 nm was examined with Multiskan MK3 (Thermo Fisher Scienti c, Inc, Waltham, MA, USA).
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