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Recombinant human apoe3

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Recombinant Human ApoE3 is a purified, recombinant form of the human apolipoprotein E3 protein. ApoE3 is a key component of certain lipoproteins and plays a role in lipid metabolism.

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Lab products found in correlation

Cyclohexane, by Merck Group (1 mentions) Igepal co 520, by Merck Group (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Lps from escherichia coli o111 b4, by Merck Group (1 mentions) Recombinant human ifn γ, by BioLegend (1 mentions) Pierce high capacity endotoxin removal spin column, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Mitotempo, by Merck Group (1 mentions) Poly 1 c tlrl pic, by InvivoGen (1 mentions) Il 13, by BioLegend (1 mentions) Methyl β cyclodextrin, by Merck Group (1 mentions) Tnf α, by BioLegend (1 mentions) Recombinant human il 10, by Thermo Fisher Scientific (1 mentions) Fsllry nh2, by Bio-Techne (1 mentions) Rwj 56110, by Bio-Techne (1 mentions) M csf, by BioLegend (1 mentions) Il 17a, by BioLegend (1 mentions)

Close spelling variants of this product

ApoE3 (6 citations)

3 protocols using recombinant human apoe3

1

Lipid-Based Delivery of miRNA for Stem Cell Regulation

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1,2‐Dioleoyl‐sn‐glycero‐3‐phosphate (sodium salt) (DOPA) and 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Cyclohexane and IGEPAL CO‐520 were provided by Sigma‐Aldrich (St Louis, MO, USA). Recombinant Human ApoE3 was synthesized by PEPROTECH (Rocky Hill, NJ, USA). All microRNAs used both in vitro and in vivo were synthesized from Ribobio (Guangzhou, China). The miR34a consisted of the antisense strand 5′‐ACAACCAGCUAAGACACUGCCA‐3′ and sense strand 5′‐UGGCAGUGUCUUAGCUGGUUGU‐3′. NC‐miRNAs have an antisense strand 5′‐CAGUACUUUUGUGUAGUACAAA‐3′ and sense strand 5′‐UUUGUACUACACAAAAGUACUG‐3′. The miRNAs labeled with fluorescent dye such as Cy5 on the antisense strand 5′ helped for imaging and tracking. SOX2 rabbit polyclonal antibody (ab97959), CXCR4 rabbit monoclonal antibody (ab181020) and Notch1 rabbit monoclonal antibody (ab52627) were purchased from Abcam (Cambridge, UK). DiI was obtained from Invitrogen (Carlsbad, CA, USA). Cell counting kit‐8 (CCK8) was provided by Dojindo Laboratories (Kumamoto, Japan). Other reagents were obtained from Sigma‐Aldrich if not specifically mentioned.
Jiang G., Chen H., Huang J., Song Q., Chen Y., Gu X., Jiang Z., Huang Y., Lin Y., Feng J., Jiang J., Bao Y., Zheng G., Chen J., Chen H, & Gao X. (2020). Tailored Lipoprotein‐Like miRNA Delivery Nanostructure Suppresses Glioma Stemness and Drug Resistance through Receptor‐Stimulated Macropinocytosis. Advanced Science, 7(5), 1903290.
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2

Recombinant Protein Characterization

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Bovine serum albumin (>96%)
was purchased from Sigma-Aldrich. Recombinant human MARCO protein
(>95%) was purchased from R&D Systems. The recombinant human
low-density
lipoprotein receptor and ApoE receptor 2 (>90%) were purchased
from
Neuromics Inc. Recombinant human ApoE3 was purchased from Peprotech
Inc.
Behan J.A., Xie Z., Wang Y.F., Yang X., Aastrup T., Yan Y., Adumeau L, & Dawson K.A. (2023). Quartz Crystal Microbalance Method to Measure Nanoparticle–Receptor Interactions and Evaluate Nanoparticle Design Efficiency. JACS Au, 3(6), 1623-1633.
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3

Endotoxin-Depleted House Dust Mite Extract Protocol

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House dust mite extract (# B84, Dermatophagoides pteronyssinus, Greer Laboratories, Inc., Lenoir, NC) was depleted of endotoxin using Pierce™ High Capacity Endotoxin Removal Spin Columns (# 88274, ThermoFisher Scientific, Waltham, MA) to generate a final concentration of 0.096 endotoxin units (EU) per microgram of HDM protein. Recombinant human IFN-γ(# 570204), TNF-α(# 570104), IL-13 (# 571104), IL-17A (# 570504), and M-CSF (# 574806) were from BioLegend (San Diego, CA). Poly (I:C) (# tlrl-pic) and polymyxin B (# tlrl-pmb) were from InvivoGen (San Diego, CA). E64 (# 5208), AEBSF (# 5175), protease activated receptor-1 (PAR-1) antagonist, RWJ 56110 (# 2614), and PAR-2 antagonist, FSLLRY-NH2 (# 4751), were from Tocris (Minneapolis, MN). Recombinant human APOE3 (# 350-02) and recombinant human IL-10 (# 200-10) were from PeproTech (Rocky Hill, NJ). The Recombinant human APOE3 contained 0.021 EU/μg of protein. Dexamethasone (# D4902), LPS from Escherichia coli O111:B4 (# L4391), MitoTEMPO (# SML0737), CA-074 methyl ester (# C5857), A-438079 hydrochloride hydrate (# A9736), E-64d (# E8640), potassium chloride (KCL) (# 60142), methyl-β-cyclodextrin (# C4555), α-cyclodextrin (# C4680) and cholesterol-loaded, water soluble, methyl-β-cyclodextrin (# C4951) were from Millipore Sigma (St. Louis MO). Z-YVAD-FMK (# 218746) and MCC950 (# 5.38120.0001) were from Calbiochem (Burlington, MA).
Gordon E.M., Yao X., Xu H., Karkowsky W., Kaler M., Kalchiem-Dekel O., Barochia A.V., Gao M., Keeran K.J., Jeffries K.R, & Levine S.J. (2019). Apolipoprotein E is a Concentration-dependent Pulmonary Danger Signal that Activates the NLRP3 Inflammasome and IL-1β Secretion by Asthmatic Macrophages. The Journal of allergy and clinical immunology, 144(2), 426-441.e3.
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