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GC1qR is a laboratory equipment product offered by Santa Cruz Biotechnology. It functions as a receptor that binds to the globular head domain of the C1q complement protein.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Traf6, by Santa Cruz Biotechnology (1 mentions) Pho irf3, by Abcam (1 mentions) Stat1, by Santa Cruz Biotechnology (1 mentions) Pcbp2, by Abcam (1 mentions) Traf3, by Santa Cruz Biotechnology (1 mentions) β actin, by Abcam (1 mentions)

2 protocols using gc1qr

1

Protein Knockdown in OKF6/TERT-2 Cells

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To knock down the levels of selected proteins, the OKF6/TERT-2 cells were grown in 6-well tissue culture plates to 50 to 80% confluence overnight. The next morning, the cells were transfected with EGFR (Santa Cruz Biotechnology; number SC-29301), WBP2 (Santa Cruz Biotechnology; number SC93955), TOLLIP (Dharmacon; number L-016930-00-0005), IFITM3 (Santa Cruz Biotechnology; number SC-97053), gC1qR (Santa Cruz Biotechnology; number SC-42880), or control (Qiagen; number 1027281) siRNA using Lipofectamine RNAiMAX (Invitrogen; number 13778150) following the manufacturer’s instructions. After 24 h posttransfection, the cells were seeded onto fibronectin-coated coverslips and incubated for another 24 h before use in the experiments. The extent of protein knockdown was determined by immunoblotting, and total loading was detected by probing the blots with an anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase) antibody (Cell Signaling; number 5174, clone D16H11).
Phan Q.T., Lin J., Solis N.V., Eng M., Swidergall M., Wang F., Li S., Gaffen S.L., Chou T.F, & Filler S.G. (2021). The Globular C1q Receptor Is Required for Epidermal Growth Factor Receptor Signaling during Candida albicans Infection. mBio, 12(6), e02716-21.
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2

Western Blot Analysis of Immune Regulators

Cited 1 time
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Western blot analysis was performed using standard procedures. Antibodies against MAVS (1:1000, Santa Cruz), IRF3, pho-IRF3 (1:1000, Abcam), NLRC3, NLRX1 (1:2000, Ting Lab (17 (link))), TRAF3 (1:200, Santa Cruz), TRAF6, Stat1 (1:500, Santa Cruz), PCBP2 and ARCH5 (1:500, Abcam), gC1qR (1:1000, Santa Cruz), β-actin (1:200,000, Abcam) were used.
Shao W.H., Shu D.H., Zhen Y., Hilliard B., Priest S.O., Cesaroni M., Ting J.P, & Cohen P.L. (2016). Prion-like MAVS aggregation in lupus patients associates with increased interferon-I. Arthritis & rheumatology (Hoboken, N.J.), 68(11), 2697-2707.
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