Bnip3 ab109362

Manufactured by Abcam

Sourced in United States, United Kingdom

BNIP3 (ab109362) is a primary antibody that detects the expression of the BNIP3 protein. BNIP3 is a pro-apoptotic member of the Bcl-2 family and plays a role in mitochondrial function and cell death pathways.

Automatically generated - may contain errors

Lab products found in correlation

Gapdh ap0063, by Bioworld Technology (2 mentions) Pgc1α sc 13067, by Santa Cruz Biotechnology (1 mentions) Lc3 1 2, by Proteintech (1 mentions) Gapdh, by Bioworld Technology (1 mentions) P62 18420 1 ap, by Proteintech (1 mentions)

Spelling variants of this product

BNIP3 (44 citations) Ab109362 (18 citations)

2 protocols using bnip3 ab109362

Muscle Protein Analysis by Western Blot

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For protein content analysis, skeletal (quadriceps femoris) and cardiac (left ventricle) muscle samples were homogenized and analyzed by Western blotting. The following antibodies were used: BNIP3 (ab109362, rabbit, 1 : 1000), p-AMPK (ab131357, rabbit, 1 : 500) from Abcam (USA); PGC-1α (sc-13067, rabbit, 1 : 200) from Santa Cruz (USA); CS (16131-1-AP, rabbit, 1 : 1000), LC 3 I/II (14600-1-AP, rabbit, 1 : 500), AMPK (10929-2-AP, rabbit, 1 : 500), DRP1 (12957-1-AP, rabbit, 1 : 500), MFN1 (13798-1-AP, rabbit, 1 : 500), and p62 (18420-1-AP, rabbit, 1 : 1000) from Proteintech (USA); and GAPDH (AP0063, rabbit, 1 : 5000) from Bioworld (USA). Membranes were analyzed and quantified using the Quantity One Imaging System (BIO-RAS, USA). Protein expression was normalized to that of GAPDH.

Dun Y., Liu S., Zhang W., Xie M, & Qiu L. (2017). Exercise Combined with Rhodiola sacra Supplementation Improves Exercise Capacity and Ameliorates Exhaustive Exercise-Induced Muscle Damage through Enhancement of Mitochondrial Quality Control. Oxidative Medicine and Cellular Longevity, 2017, 8024857.

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Cardiac Protein Expression Analysis

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Proteins were extracted from the heart, the following antibodies were used: BNIP3 (ab109362, rabbit, 1:1000) from Abcam (England); LC3-II and LC3-I (14600-1-AP, rabbit, 1:500), and P62 (18420-1-AP, rabbit, 1:1000) from Proteintech (USA); HSP70 (21206-1-AP, rabbit,1:500) from Proteintech (USA) and GAPDH (AP0063, rabbit, 1:5000) from Bioworld (USA); Protein expression was normalized to that of GAPDH.

Jiang L., Shen X., Dun Y., Xie M., Fu S., Zhang W., Qiu L., Ripley-Gonzalez J.W, & Liu S. (2021). Exercise combined with trimetazidine improves anti-fatal stress capacity through enhancing autophagy and heat shock protein 70 of myocardium in mice. International Journal of Medical Sciences, 18(7), 1680-1686.

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