Agilent 1260 lc instrument

Crude PHP (S27812) was extracted from the fermentation broth of Paecilomyces hepiali that purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The crude PHP solution was repeatedly deproteinated by the sevage method [18 (link)] and purified by ethanol precipitation [52 (link)]. The molecular size distribution of PHP was analyzed by Agilent 1260 LC instrument (Agilent Technologies, Santa Clara, CA, USA) with a TSKgel GMPW_XL analysis column (7.8 mm × 300 mm, Tosoh, Tokyo, Japan). The MW of the PHP was calculated according to the calibration curve established by the dextran standards (T-10, T-40, T-70, T-500, and T-2000). The monosaccharide composition of PHP was analyzed by high-performance liquid chromatography (HPLC) via Agilent 1260 LC instrument (Agilent Technologies Inc, Santa Clara, CA, USA) with a ZORBAX Eclipse XDB-C18 column (id 5 µm, 4.6 × 250 mm, Agilent Technologies Inc., Santa Clara, CA, USA) according to the monosaccharide standard, including rhamnose, arabinose and galactose, etc. The Fourier transforms infrared spectroscopy (FT-IR) spectrum of PHP was detected with a FT-IR spectrometer (Alpha type, Bruker, Billerica, MA, USA). PHP was processed by KBr pressure disk technology and measured within the frequency range of 4000~400 cm⁻¹.