Gfp filter

Manufactured by Leica

Sourced in Germany

The GFP (Green Fluorescent Protein) filter is a specialized optical filter designed to isolate and detect the emission of green fluorescent light. It is a core component of various imaging and analysis systems used in scientific research and applications. The GFP filter selectively transmits the specific wavelength range associated with the green fluorescent signal, allowing researchers to visualize and study fluorescently labeled samples with high specificity and sensitivity.

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Lab products found in correlation

Dm6000b, by Leica (1 mentions) Plate spectrophotometer, by PerkinElmer (1 mentions) Dfc480, by Leica (1 mentions) Dm6000b microscope, by Leica (1 mentions) R software v4, by R Project for Statistical Computing (1 mentions) Dm6b fluorescence microscope, by Leica (1 mentions) Hbo 100 mercury lamp, by Leica (1 mentions)

Spelling variants of this product

GFP filter cube (4 citations) GFP filter set (3 citations) ET-GFP and TXR LP filters (2 citations) ET GFP band pass filter cube (2 citations) ET GFP Filter set (2 citations)

5 protocols using gfp filter

Fluorescein Liposomes Skin Absorption

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Fluorescein liposomes skin absorption was analyzed by collecting fluorescein samples directly from the receptor medium of Franz cells, protected from light, and measuring the concentration by fluorometry with a plate spectrophotometer (PerkinElmer Inc., Waltham, MA, USA; victor 3). A standard curve was constructed with known concentrations of fluorescein to calculate fluorescein concentration as μg/cm² of skin.
A Fluorescence Microscope Leica^® DM6000B, equipped with a photographic camera DFC480 and a Leica GFP filter, was used to monitor fluorescein liposomes skin absorption. Excitation of the sample was carried out with 470/40 nm blue light. The fluorescent emission obtained was of 525/50 nm, in green light.

Serrano G., Almudéver P., Serrano J.M., Milara J., Torrens A., Expósito I, & Cortijo J. (2015). Phosphatidylcholine liposomes as carriers to improve topical ascorbic acid treatment of skin disorders. Clinical, Cosmetic and Investigational Dermatology, 8, 591-599.

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Quantitative Hair Follicle Fluorescence

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A Leica DM6000B microscope (Wetzlar, Germany), equipped with a digital camera DFC480 and a Leica GFP filter was used for the fluorescence assessment. Excitation of the sample was carried out with 470+40 nm blue light. The fluorescent emission obtained was in the visible green waveband at 525+50 nm. Assessment of the fluorescence intensity at the two time periods was determined by comparing images. Evaluation was done by scoring specimens from 0 to 8 (adapted from the Ashcroft scale for pulmonary fibrosis) to determine the amount of product present in the specimens corresponding to each of the time frames. A score of 0 indicates no staining of the follicular duct (nonexposed hair follicle). A value of 8 means a totally pigmented hair follicle.

Serrano G., Almudéver P., Serrano J.M., Cortijo J., Faus C., Reyes M., Expósito I., Torrens A, & Millán F. (2015). Microneedling dilates the follicular infundibulum and increases transfollicular absorption of liposomal sepia melanin. Clinical, Cosmetic and Investigational Dermatology, 8, 313-318.

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Fungal Inhibition by Rpv12 Locus Analysis

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Mycelial development was followed to study the inhibition of pathogen progress mediated by the Rpv12 locus after 72 hpi by comparison of genotypes Gf.43-21, Gf.99-03, and 65-153-18. The cultivar “Italia” served as susceptible control. For every genotype under study, three leaves were taken and out of every leaf three leaf discs were stained with alkaline aniline blue (see above). Of every leaf disc, five pictures of 100× magnification were taken using a fluorescence microscope with GFP-Filter (Leica A). In total, 45 images were taken at 72 hpi from every genotype and analyzed (Müllner and Zyprian, 2022 (link)). The area of mycelium was determined using Welch’s-T-Test and R Software v4.0.3 (Team, 2018 ) (https://www.R-project.org/).

Frommer B., Müllner S., Holtgräwe D., Viehöver P., Huettel B., Töpfer R., Weisshaar B, & Zyprian E. (2023). Phased grapevine genome sequence of an Rpv12 carrier for biotechnological exploration of resistance to Plasmopara viticola. Frontiers in Plant Science, 14, 1180982.

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Tunicamycin-Induced ER Stress Assay in C. elegans

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Tunicamycin is a chemical that inhibits N-linked glycosylation, leading to an accumulation of misfolded proteins in the endoplasmic reticulum (ER) [33 (link)]. To induce stress in C. elegans, synchronized L1 larvae of the SJ4005 (hsp-4::gfp) strain were grown in various concentrations of DOP from the three sources and the control. On days 1, 3, and 4 of adulthood, 50 worms were placed in a 25 ng/µL tunicamycin M9 buffer and incubated at 20 °C for 4 h [34 (link)]. The worms were then immobilized with 5 µM levamisole on a 2% agarose pad on a glass slide, covered with a coverslip, and imaged using a DM6B fluorescence microscope with the GFP filter (Leica, Wetzlar, Germany). The fluorescence intensity was quantified using ImageJ software [35 (link)]. The assay was performed in triplicate for three independent trials.

Okoro N.O., Odiba A.S., Yu Q., He B., Liao G., Jin C., Fang W, & Wang B. (2023). Polysaccharides Extracted from Dendrobium officinale Grown in Different Environments Elicit Varying Health Benefits in Caenorhabditis elegans. Nutrients, 15(12), 2641.

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Plasma Membrane Permeability Assay

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Cells with a plasma-membrane permeabilized were discriminated using the membrane-impermeant SYTOX Green (SG) probe, as previously described (Machado and Soares, 2012a) . SG enters only in cells with compromised membrane and binds to DNA, exhibiting a green fluorescence (Haugland, 2005) .
Cells (1 £ 10 6 mL -1 ) were incubated with 0.5 μmol L -1 SG (Molecular Probes, Invitrogen), in the dark, at room temperature, for 20 min. Positive (cells with permeabilized membrane by heat treatment, at 65 • C, for 1 h) and negative control (cells not exposed to TCS) were used. Cells were observed with an epifluorescence microscope, equipped with an HBO-100 mercury lamp and a GFP filter from Leica. The experiment was repeated, independently, three times. In each experiment and for each TCS concentration, at least 400 cells were examined; therefore, for each TCS concentration, a total of ≥ 1200 cells were analyzed in randomly selected microscope fields.

Machado M.D, & Soares E.V. (2021). Toxicological effects induced by the biocide triclosan on Pseudokirchneriella subcapitata. Aquatic toxicology (Amsterdam, Netherlands), 230.

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