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Anti mlkl

Manufactured by BD
Sourced in United States

Anti-MLKL is a laboratory reagent used for research purposes. It functions as an antibody that specifically binds to the MLKL (Mixed Lineage Kinase Domain-Like Protein) protein, which is involved in the regulation of programmed cell death pathways. The core function of Anti-MLKL is to enable the detection and study of MLKL in various experimental settings.

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2 protocols using anti mlkl

1

Necroptosis Pathway Protein Analysis

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Fisetin and z-VAD were purchased from Sigma, USA. The primary antibodies used in this study for Western blotting were as follows: anti-HMGB1 (Invitrogen, NY, USA), 1:1000 dilution; anti-ZBP1 (R&D system, USA), 1:1000 dilution; anti-RIP3 (Calbiochem, San Diego, CA), 1:1000 dilution; anti-RIP1 (Calbiochem, San Diego, CA), 1:1000 dilution; anti-MLKL (BD Biosciences, USA), 1:1000 dilution; and anti-GAPDH (Santa Cruz, CA, USA), 1:5000 dilution.
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2

Targeted Cell Death Mechanism Analysis

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Fisetin and z-VAD were purchased from Sigma, USA. The primary antibodies used in this study for western blotting were as follows: anti-HMGB1(Invitrogen, NY, USA), 1:1000 dilution; anti-ZBP1 (R&D system, USA), 1:1000 dilution; anti-RIP3(Calbiochem, San Diego, CA), 1:1000 dilution; anti-RIP1(Calbiochem, San Diego, CA), 1:1000 dilution; anti-MLKL (BD Biosciences, USA), 1:1000 dilution; anti-GAPDH (Santa Cruz, CA, USA), 1:5000 dilution.
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