Tg(kdrl:eGFP) zebrafish was provided by Dr Stainier (Max Planck Institute for Heart and Lung Research). Embryos that were derived from the line were maintained in E3 medium containing 200 μM 1-phenyl-2-thiourea (P7629, Sigma-Aldrich, St Louis, MO). Approximately 100–400 RFP-labelled MBA-MB-231 or MIA-PaCa2 cells were injected into the duct of Cuvier of the zebrafish at 2 dpf. The fish were randomly assigned to two groups. One group was maintained in the presence of pizotifen-containing E3 medium and the other group was maintained in vehicle-containing E3 medium.
1 phenyl 2 thiourea p7629
Manufactured by Merck Group
1-phenyl-2-thiourea (P7629) is a laboratory reagent used in various chemical and biochemical applications. It serves as a precursor or intermediate in organic synthesis. The product specification and safety information are available upon request.
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Lab products found in correlation
3 protocols using 1 phenyl 2 thiourea p7629
1
Zebrafish Model of Metastatic Cancer
2
Zebrafish Lines and Pigmentation Prevention
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A list of zebrafish lines used in this work is provided in the Supplementary Table 7 . To prevent the pigmentation in embryos older than 24 hpf, 0.003% 1-phenyl-2-thiourea (P7629, Sigma-Aldrich) in 0.3 × Danieau's solution (17.4 mM NaCl, 0.21 mM KCl, 0.12 mM MgSO4·7H2O, 0.18 mM Ca(NO3)2, 1.5 mM Hepes) was used. The zebrafish were handled according to the regulations of the state of North Rhine-Westphalia, supervised by the veterinarian office of the city of Muenster.
3
Zebrafish Embryo Maintenance and Transgenic Lines
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Wild-type, transgenic and mutant zebrafish embryos were raised at 28°C in Embryo Water (Volvic© water containing 0.28 mg/mL Methylene Blue [M-4159; Sigma] and 0.03 mg/mL 1-phenyl-2-thiourea [P-7629; Sigma]), and staged according to Westerfield (Westerfield, 2007) . The moonshine NQ039 or t30813 (Demy et al., 2017) and moonshine TB222 (Ransom et al., 2004) mutations were maintained by outcross of heterozygous carriers with WT and/or Tg fish from the following lines: Tübingen wildtype (WT) fish (ZIRC), Tg(mpeg1:mCherry-F) ump2 (Nguyen-Chi et al., 2014) , Tg(lyz:DsRed2) nz50 (Hall et al., 2007) , Tg(mfap4:mCherry-F) ump6 (Phan et al., 2018) , Tg(mpeg1:Gal4FF) gl25 (Palha et al., 2013) , Tg(UAS:Kaede) rk8 (Hatta et al., 2006) , Tg(mfap4:turquoise) xt27 (Walton et al., 2015) , Tg(UAS-E1b:Eco.NfsB-mCherry) c264 (Davison et al., 2007) , Tg(gata2b:Gal4;UAS:lifeAct-eGFP) (Butko et al., 2015) , Tg(mpeg:GFP-F) sh425 (Keatinge et al., 2015) , Tg(mpx:GFP) i114 (Renshaw et al., 2006) , Tg(gata1:dsRed) sd2 (Traver et al., 2003) , and Tg(CD41:GFP) la2 (Lin et al., 2005) .
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