Nrf2 dna binding elisa kit

All kits used for this part were rat-specific. MDA ELISA kit for rats was used to measure levels of malondialdehyde (MDA) in the liver homogenates (#MBS268427, MyBioSource, CA, USA). GSH ELISA kit (#MBS265966; MyBioSource, CA, USA), SOD ELISA kit (#MBS036924, MyBioSource, CA, USA), CAT ELISA kit (#MBS726781, MyBioSource, CA, USA), IL-6 ELISA kit (#MBS269892, MyBioSource, CA, USA), HO-1 ELISA kit (MBS764989, MyBioSource, CA, USA) and TNF-αELISA kits (#MBS2507393, MyBioSource, CA, USA) were used to measure the levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the liver homogenates. G6PC ELISA kit (#MBS097902 MyBioSource, CA, USA), FBP1 ELISA kit (#MBS931493, MyBioSource, CA, USA), and GLY ELISA kit (#MBS1600418; MyBioSource, CA, USA) were used to measure the levels of glucose-6 phosphatase (G-6-Pase), fructose-1,6-bisphosphatase 1 (PBP1), and glycogen in the liver homogenates. The TransAM Nrf2, NF-E2-related factor 2 (Nrf2 DNA binding ELISA) kit (#50296, ActiveMotif, Tokyo, Japan) and TransAM NF-κB p65 ELISA kits (#40096, ActiveMotif, Tokyo, Japan) were used to measure the total and cytoplasmic levels of Nrf2 and NF-κB p65 in the homogenates. All procedures were conducted according to each manufacturer’s instructions for 8 samples/groups.

Nuclei were extracted from the left ventricle of the heart and SKM tissues using the Nuclear Extract Kit (Active Motif, Carlsbad, CA) as per manufacturer's instructions. Nrf2 activity was measured in nuclear extracts by an Nrf2‐DNA‐binding ELISA kit (Active Motif) as we have reported before (Singh et al., 2015).