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2 protocols using ferrous ammonium sulfate hexahydrate

1

Antioxidant Capacity of Pullulan-Based Composites

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Bovine serum albumin (BSA), epigallocatechin gallate (EGCG), anhydrous ferric chloride, potassium ferricyanide, ferrous ammonium sulfate hexahydrate, salicylic acid, trichloroacetic acid, 1,1-diphenyl-2-Picrylhydrazine (DPPH), and 2,2′-Diaza-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Pullulan (PUL) was purchased from Shanghai Yien Chemical Technology Co., Ltd. (Shanghai, China). Hydrogen peroxide (purity ≥ 30%), potassium dihydrogen phosphate, dipotassium hydrogen phosphate, absolute ethanol, hydrochloric acid, and sodium hydroxide were purchased from China National Medicines Corporation Ltd. (Shanghai, China).
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2

Synthesis and Characterization of PEG-based Biopolymer

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Poly(ethylene glycol) methacrylate (PEG, Mn 360 g/mol), (3-mercaptopropyl) trimethoxysilane (MPS), TBNO, sodium dodecyl sulfate (SDS), IPA, ethanol, acetone and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich and used without further purification. Ferrous ammonium sulfate hexahydrate and 4% paraformaldehyde were purchased from Macklin Inc. and Santa Cruz Biotechnology, Inc. respectively. Deionized (DI) water was from a Milli-Q water system. Antibiotic/Antimycotic solution (10,000 U/mL Penic illin, 10,000 μg/mL Streptomycin, 25 μg/mL Amphotericin B) and PBS was from GE Healthcare Life Science. 00.25% Trypsin-EDTA, fetal bovine serum (FBS) and Dulbecco's Modified Eagle's Medium (DMEM) were from Thermo Fisher Scientific. Bacterial growth media and agar were all purchased from BD. Strains used, MRSA BAA38, MRSE 35984, VRE 583, A. baumannii AB-1, E. coli UTI89 and P. aeruginosa PAO1, were obtained from ATCC.
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