Cation-exchange chromatography (CEX) was used to separate charge variants of PF-06439535. CEX separation was performed using a Dionex ProPac WCX-10 cation exchange column (4 mm × 250 mm, 10 micron, Thermo Fisher Scientific) on a Waters HPLC system (Waters Corporation), and the separation was monitored with UV detection at a wavelength of 214 nm.
Dionex propac wcx 10
Manufactured by Thermo Fisher Scientific
The Dionex ProPac WCX-10 is a strong cation-exchange chromatography column designed for the separation and purification of biomolecules, such as proteins, peptides, and oligonucleotides. The column features a polymeric resin with a high surface area and strong cation-exchange capacity, which enables efficient and selective separation of analytes based on their charge properties.
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Lab products found in correlation
2 protocols using dionex propac wcx 10
1
Cation-exchange Chromatography for Protein Separation
2
Charge Variant Separation of FKB327 and Adalimumab
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A CE chromatography‐based HPLC method was used to separate charge variants in FKB327 and adalimumab with a Dionex™ ProPac™ WCX‐10 (Thermo Fisher Scientific) with a sodium chloride gradient in sodium phosphate buffer, pH 6.5. Eluted peaks were detected at 280 nm.
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