Re co 5cl

RuCl₃·nH₂O (J&K), bpy (J&K), phen (J&K), DIP (J&K), Re(CO)₅Cl (Sigma Aldrich), silver trifluoromethanesulfonate (Alfa Aesar), 4-pyridinecarboxaldehyde (Alfa Aesar), 5,6-diamino-1,10-phenanthroline (Alfa Aesar), NH₄PF₆ (Alfa Aesar), 4% paraformaldehyde (Beyotime), crystal violet (Beyotime), Dulbecco’s Modified Eagle Medium (DMEM, Gibco), Roswell Park Memorial Institute 1640 (RPMI 1640, Gibco), Fetal bovine serum (FBS, Gibco), penicillin-streptomycin (Gibco), MTT (J&K), Rh123 (J&K), H₂DCFDA (J&K), Hoechst 33342 (J&K), Annexin V-FITC Apoptosis Detection Kit (Beyotime). Primary antibodies against caspase-3 and PARP were purchased from Cell Signaling Technology. RuRe-1, RuRe-2 were dissolved in DMSO just before the experiments, and the concentration of DMSO in biological experiments was 1% (v/v). Cisplatin was dissolved in 0.9% sodium chloride solution just before use.
A LCQ DECA XP spectrometer was used for obtaining ESI-MS spectra. A Bruker Avance 600 spectrometer was used for obtaining ¹H NMR spectra. A SpetraMax M2 plate reader was used for determining cell viability. A Nikon A1R/A1 laser-scanning confocal microscope was used for obtaining cell imaging images. A CyFlow Space flow cytometer was used for performing the flow cytometry analysis.

Melamine, benzene-1,3,5-tricarbaldehyde, and Re(CO)₅Cl were obtained from Sigma-Aldrich and used as received, without further purifications. All manipulations of air and moisture sensitive materials were conducted under a nitrogen atmosphere on a dual manifold Schlenk line. The glassware was oven-dried prior to use. DMSO (99.5% Sigma Aldrich, St. Louis, MO, USA) was degassed while toluene (99.5% Sigma-Aldrich) was distilled on Na(s) and CH₂Cl₂ on P₂O₅, both under a N₂ atmosphere.

Commercially available pentacarbonyl chlororhenium(I), Re(CO)₅Cl (Sigma Aldrich), poly(9-vinylcarbazole) (PVK, M_n = 25,000–50,000; Sigma Aldrich), poly(3,4-(ethylenedioxy)thiophene): poly-(styrenesulfonate) (PEDOT:PSS, 0.1–1.0 S/cm, Sigma Aldrich), substrates with pixilated ITO anodes (Ossila) and solvents for synthesis (of reagent grade) and for spectroscopic studies (of spectroscopic grade) were used as received without further purification. All aryl-substituted 2,6-di(thiazol-2-yl)pyridine (Ar¹-dtpy – Ar⁶-dtpy) were prepared according to the method reported in our previous work [34 (link)].