Ab98933

Total protein was extracted and protein concentration was detected by BCA assay. Western blot analysis was performed as previously described [39 (link)]. EFNB2 (ab69858, Abcam, Cambridge, UK), EPHB4 (ab150545, ab98933, Abcam), LDLR (ab52818, Abcam), β-catenin (ab32572, Abcam), VLDLR (ab203271, Abcam), SCARB1(ab52629, Abcam), STAT3(ab68153, Abcam), p-STAT3(ab267373, Abcam), JAK2(ab108596, Abcam), p-JAK2(ab108596, Abcam), SREBP2(ab30682, Abcam), proliferating cell nuclear antigen (PCNA; Proteintech Group, Inc., Sankt Leon-Rot, Germany) primary antibodies were used. Horseradish peroxidase (HRP)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (SA00001-1) were obtained from Proteintech Group, Inc (Chicago, US).

Total protein was extracted using radioimmunoprecipitation assay (RIPA) buffer supplemented with 1% protease inhibitors and phosphatase inhibitors. Protein concentration was measured with the bicinchoninic acid (BCA) assay. Western blot analysis was performed as previously described^{12 (link)}. NPTX2 (ab69858, Abcam), FZD6 (ab150545, ab98933, Abcam), β-catenin (ab32572, Abcam), MYC (ab39688, Abcam), cyclin D1 (CCND1; ab16663, Abcam), snail-1 (ab53519, Abcam), N-cadherin (ab76011, Abcam), E-cadherin (ab1416, Abcam), Ki-67, and proliferating cell nuclear antigen (PCNA; Proteintech Group, Inc.) primary antibodies were used. Horseradish peroxidase (HRP)-conjugated Affinipure goat anti-rabbit IgG (H + L) and HRP-conjugated Affinipure goat anti-mouse IgG (H + L) were obtained from Proteintech Group, Inc (Jackson).