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Purified anti human cd28 cd28.2

Manufactured by Thermo Fisher Scientific

Purified anti-human CD28 (CD28.2) is a monoclonal antibody that specifically binds to the CD28 receptor on human T cells. CD28 is a costimulatory molecule that plays a crucial role in T cell activation and survival. This antibody can be used for flow cytometry, immunohistochemistry, and other immunological applications to detect and study CD28-expressing cells.

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2 protocols using purified anti human cd28 cd28.2

1

Regulatory T Cell Induction Protocol

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Purified anti-human CD3 (OKT3) and purified anti-human CD28 (CD28.2) were purchased from eBioscience. All fluorochrome-conjugated antibodies (anti-human CD4 (RPA-T4), anti-human CD45RA (HI100), anti-human CD25 (BC96), anti-human FoxP3 (PCH101), anti-human IFNγ, anti-human IL-4, anti-human IL-17, and anti-mouse IL-6 were from eBioscience. Recombinant human IL-2 (202-IL), human TGFβ1 (240-B), and human latent TGFβ1 (299-LT) were purchased from R&D Systems. Anti-TGFβ (1D11.16.8), anti-CD25 (PC-61.5.3), and their isotype control antibodies (MOPC-21, HRPN) were from Bio X Cell. PGE2, TGFβ, and IL-6 ELISA Ready-SET-Go! kits were purchased from eBioscience.
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2

Detailed Immunology Protocol with Antibodies

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Purified anti-human CD3 (OKT3) and purified anti-human CD28 (CD28.2) were from eBioscience. Anti-human Bcl6 (EP529Y) was from abcam (Cambridge, MA, USA). Anti-CBP (D6C5) and anti-P300 (E6D1T) were from Cell Signal Technology (USA). Anti-β-actin (AB21800) and HRP-conjugated anti-rabbit and anti-mouse IgG (ABL3012 and ABL3032) were from Absci (Baltimore, MD, USA). Anti-β-hydroxybutyryllysine antibody (PTM-1204) was from PTMbio (China). Protein A/G-PLUS-agarose beads (37478) were from Cell Signaling Technology (Danvers, USA). Fluorochrome-conjugated anti-CD4 (RPA-T4), anti-human CD185 (RF8B2), anti-human CD279 (MIH4), and anti-human CD278 (DX29) were from BD Bioscience. Recombinant human IL-12, human TGFβ, human IL-23, and human IL-6 were from R&D Systems.
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