The largest database of trusted experimental protocols

2 protocols using phospho tyrosine antibody

1

Antibody Generation and Immunoblotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit polyclonal polyclonal antibodies recognizing pan-PIPKIγ or Y639-phosphorylated PIPKIγ were generated as described previously [5 (link)]. HA antibody is from Millipore. Antibodies against phosphorylated ERK1/2, total ERK1/2, phosphorylated Akt, total Akt, phosphorylated STAT3, total STAT3 are from Cell Signaling. β-actin and talin antibodies are from Sigma. Phospho-tyrosine antibody is from BD Biosciences. Alexa Flour 555-conjugated phalloidin, Alexa Flour 488-conjugated goat anti-mouse antibody, Alexa Flour 555-conjugated goat anti-rabbit antibody and Alexa Fluor 555-conjugated goat anti-rat antibody are from Molecular probes.
+ Open protocol
+ Expand
2

Antibody Generation and Immunoblotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit polyclonal polyclonal antibodies recognizing pan-PIPKIγ or Y639-phosphorylated PIPKIγ were generated as described previously [5 (link)]. HA antibody is from Millipore. Antibodies against phosphorylated ERK1/2, total ERK1/2, phosphorylated Akt, total Akt, phosphorylated STAT3, total STAT3 are from Cell Signaling. β-actin and talin antibodies are from Sigma. Phospho-tyrosine antibody is from BD Biosciences. Alexa Flour 555-conjugated phalloidin, Alexa Flour 488-conjugated goat anti-mouse antibody, Alexa Flour 555-conjugated goat anti-rabbit antibody and Alexa Fluor 555-conjugated goat anti-rat antibody are from Molecular probes.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!