Anti symplekin

Anti-HA (clone 3F10, Sigma-Aldrich), anti-α-Tubulin (clone DM1A, Sigma-Aldrich), anti-Cdk1 (ab18, Abcam, Cambridge, UK), anti-ERK1/ERK2 (9102, Cell Signaling, Danvers, MA), anti-xGLD2 (rabbit polyclonal antibody raised against KRRSDEGNSPYDVKC peptide, X. laevis), anti-4E-T (2297, Cell Signaling), anti-DDX6 (PD009, MBL, Nagoya, Japan), anti-hCPEB4 for WB (rabbit polyclonal antibody raised against amino acids 1–302), anti-hCPEB4 for IP (ab83009, Abcam), anti-phospho Histone H3 (Ser 10) (06–570, Merck Millipore, Billerica, MA), anti-Symplekin (610644, BD Transduction Laboratories, Franklin Lakes, NJ), anti-CPSF2 (ab126760, Abcam), anti-PARN (ab89831, Abcam), anti-hGLD2 (AP5092, Abgent, San Diego, CA), anti-S6 (2317, Cell Signaling), anti-Vinculin (ab18058, Abcam).

The anti-calgranulin B (Santa Cruz Biotechnology, Dallas, TX, USA), anti-symplekin (BD Transduction Laboratories, San Jose, CA, USA), anti-FLAG (anti-DYKDDDDK; BioLegend, San Diego, CA, USA), anti-flotillin-1 (Abcam, Cambridge, UK), anti-dynein intermediate chain 1 (DYNC1) (Abcam), anti-CD59 (Abcam), and anti-β-actin (Abcam) antibodies were used for western blot (WB) analyses. Horseradish peroxidase-conjugated anti-rabbit (Vector Laboratories, Burlingame, CA, USA), anti-mouse (Vector Laboratories), and anti-rat immunoglobulin (Santa Cruz Biotechnology) secondary antibodies were used.