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Bl473t8 150fc

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The BL473T8-150FC is a laser module that produces a wavelength of 473 nm. It has a rated output power of 150 mW and a fiber-coupled design.

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Lab products found in correlation

Power meter, by Thorlabs (2 mentions) Labview, by National Instruments (2 mentions)

3 protocols using bl473t8 150fc

1

Chronic Optogenetic Stimulation in Mice

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In addition to the implants for recording neural signals, a 0.39 NA, 400 μm optical fiber (1 mm fiber length, ThorLabs) was chronically implanted in the nasal cavity for optogenetic stimulations. Mice were stimulated in their home cage using a 473 nm laser (SLOC Lasers, BL473T8-150FC) coupled to an articulated rotary joint patch cable (ThorLabs). For most experiments, stimulations were comprised of five 150-ms pulses at 0.5 Hz delivered through a protocol written in LabView (National Instruments). When testing the effects of stimulation length, five pulses of length 5, 50, and 150 ms were used. If not otherwise stated, laser stimulation was at the highest intensity that the mouse consistently slept through (ranging from 5 to 30 mW/mm2). On the first recording day, the stimulus was titrated for each mouse. If the mouse repeatedly woke up to a stimulus intensity (i.e., more than three times), the laser power was lowered. If the mouse did not wake up to multiple stimulations at that intensity, the laser power was increased. Laser intensity was measured at the output from the patch cable using a power meter (ThorLabs) and ranged from 0.7 to 30 mW/mm2.
Schreck M.R., Zhuang L., Janke E., Moberly A.H., Bhattarai J.P., Gottfried J.A., Wesson D.W, & Ma M. (2022). State-dependent olfactory processing in freely behaving mice. Cell reports, 38(9), 110450.
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2

Optogenetic Stimulation of Nasal ChR2 Mice

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OMP-ChR2 mice with optic fibers implanted in their nasal cavities were stimulated in their home cage using a 473 nm laser (SLOC Lasers, BL473T8-150FC) coupled to an articulated rotary joint patch cable (Thor Labs). Laser light power was adjusted to achieve an intensity of ~15 mW at the fiber tip (continuous output) and the laser was controlled via delivery of 0–5 V TTL pulses generated by an arbitrary waveform generator (Agilent, 33201 A).
Moberly A.H., Schreck M., Bhattarai J.P., Zweifel L.S., Luo W, & Ma M. (2018). Olfactory inputs modulate respiration-related rhythmic activity in the prefrontal cortex and freezing behavior. Nature Communications, 9, 1528.
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3

Chronic Optogenetic Stimulation in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
In addition to the implants for recording neural signals, a 0.39 NA, 400 μm optical fiber (1 mm fiber length, ThorLabs) was chronically implanted in the nasal cavity for optogenetic stimulations. Mice were stimulated in their home cage using a 473 nm laser (SLOC Lasers, BL473T8-150FC) coupled to an articulated rotary joint patch cable (ThorLabs). For most experiments, stimulations were comprised of five 150-ms pulses at 0.5 Hz delivered through a protocol written in LabView (National Instruments). When testing the effects of stimulation length, five pulses of length 5, 50, and 150 ms were used. If not otherwise stated, laser stimulation was at the highest intensity that the mouse consistently slept through (ranging from 5 to 30 mW/mm2). On the first recording day, the stimulus was titrated for each mouse. If the mouse repeatedly woke up to a stimulus intensity (i.e., more than three times), the laser power was lowered. If the mouse did not wake up to multiple stimulations at that intensity, the laser power was increased. Laser intensity was measured at the output from the patch cable using a power meter (ThorLabs) and ranged from 0.7 to 30 mW/mm2.
Schreck M.R., Zhuang L., Janke E., Moberly A.H., Bhattarai J.P., Gottfried J.A., Wesson D.W, & Ma M. (2022). State-dependent olfactory processing in freely behaving mice. Cell reports, 38(9), 110450.
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