Total mononuclear cell suspension derived from peripheral blood and pleural effusion samples was obtained by ficoll hystopaque (Lonza, Basel, Switzerland) gradient stratification [25 (link)]. To identify NK cell subsets, cells obtained were subsequently stained with monoclonal antibodies against surface markers (CD14-PE and CD45-FITC, CD3-PerCP, CD56-APC, CD16-FITC, CD9-PE, CD49a-PE, CD57-PE, CD69-PE, NKp30-PE, NKG2D-PE, and NKG2A-PE) all purchased from Miltenyi Biotec (Auburn, CA) and analysed by a Becton Dickinson (BD) FACSCanto II flow cytometer (Becton Dickinson, CA). Briefly, after physical parameter analysis (FSC/SSC), CD45+CD14− lymphocytes were gated and assessed for NK markers. NK cells were gated on CD45+CD3−CD56+ total lymphocytes.
Nkp30 pe
Manufactured by Miltenyi Biotec
NKp30-PE is a laboratory reagent used to detect and analyze natural killer (NK) cells. It is a fluorescently labeled antibody that specifically binds to the NKp30 receptor on the surface of NK cells, enabling their identification and quantification through flow cytometry or other analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Nkg2d pe, by Miltenyi Biotec (2 mentions)
Cd57 pe, by Miltenyi Biotec (1 mentions)
Cd45 fitc, by Miltenyi Biotec (1 mentions)
Cd16 fitc, by Miltenyi Biotec (1 mentions)
Cd56 apc, by Miltenyi Biotec (1 mentions)
Cd69 pe, by Miltenyi Biotec (1 mentions)
Cd3 percp, by Miltenyi Biotec (1 mentions)
Nkg2a pe, by Miltenyi Biotec (1 mentions)
Cd14 pe, by Miltenyi Biotec (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Cd3 viogreen, by Miltenyi Biotec (1 mentions)
Pd 1 percp, by Miltenyi Biotec (1 mentions)
Nkg2a apc, by Miltenyi Biotec (1 mentions)
Cd45 percp, by Miltenyi Biotec (1 mentions)
Cd8 vioblue, by Miltenyi Biotec (1 mentions)
Red blood cell lysis solution, by Miltenyi Biotec (1 mentions)
Cd4 pe, by Miltenyi Biotec (1 mentions)
Cd56 apc vio770, by Miltenyi Biotec (1 mentions)
2 protocols using nkp30 pe
1
Profiling NK Cell Subsets from Peripheral Blood and Pleural Effusion
2
Comprehensive Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies (CD8 Vioblue, CD3 Viogreen, vγ9 vδ2 FITC, CD4 PE, CD45 Per-CP, vγ9 vδ1 APC, CD56 APC-Vio770, CD16 Vioblue, NKG2D PE, PD-1 Per-CP, CD158b PE-Vio770, NKG2A APC, TCR-γδ APC-Vio770, NKp30 PE) (Miltenyi Biotec, San Diego, CA) were added to 25 µL of whole blood at the proper titrated volumes and allowed to incubate for 30 minutes at room temperature in the dark. Blood samples were lysed with 1X Red Blood Cell Lysis solution (Miltenyi Biotec, San Diego, CA), washed twice in phosphate-buffered saline (PBS), and resuspended in 200 µL PBS.
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