Ascorbic acid content was determined according to the previous report (1 (link)). Fifty milligram of sample powder was extracted with 5 mL 1.0% oxalic acid, subsequently centrifuged 5 min at 4,000 g. Each sample was filtered through a 0.45 μm cellulose acetate filter. HPLC analysis of ascorbic acid was carried out using an Agilent 1260 instrument with a variable wavelength detector (VWD) detector (Agilent Technologies, Inc., Palo Alto, USA). Sample were separated on a Waters Spherisorb C18 column (150 × 4.6 mm i.d.; 5 μm particle size), using a solvent of 0.1% oxalic acid at a flow rate of 1.0 mL min−1. The amount of ascorbic acid was calculated from absorbance values at 243 nm, using authentic ascorbic acid (Sangon Biotech Co., Ltd., shanghai, China) as a standard. Result of ascorbic acid content was expressed as mg g−1 of dry weight.
Authentic ascorbic acid
Manufactured by Sangon
Sourced in China
Authentic ascorbic acid is a pure chemical compound, also known as vitamin C. It is a white, crystalline powder that is highly soluble in water. Ascorbic acid is an essential nutrient required for various physiological functions in the human body.
Automatically generated - may contain errors
3 protocols using authentic ascorbic acid
1
Ascorbic Acid Content Determination
2
HPLC Determination of Ascorbic Acid
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Fifty mg of sample powder was extracted with 5 mL 1.0% oxalic acid, subsequently centrifuged 5 min at 4000g. Each sample was filtered through a 0.45 µm cellulose acetate filter. HPLC analysis of ascorbic acid was carried out using an Agilent 1260 instrument with a VWD detector (Agilent Technologies, Inc., Palo Alto, USA). Samples were separated on a Waters Spherisorb C18 column (150 mm × 4.6 mm i.d.; 5 µm particle size), using a solvent of 0.1% oxalic acid at a flow rate of 1.0 mL min−1. The amount of ascorbic acid was calculated from absorbance values at 243 nm, using authentic ascorbic acid (Sangon Biotech Co., Ltd., shanghai, China) as a standard. Result of ascorbic acid content was expressed as g kg−1 of dry weight (Sun et al., 2018 (link)).
3
Quantifying Ascorbic Acid Content
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Ascorbic acid content was determined according to the previous report (17 (link)). Fifty mg of sample powder was extracted with 5 mL 1.0% oxalic acid, subsequently centrifuged 5 min at 4,000 g. Each sample was filtered through a 0.45 μm cellulose acetate filter. HPLC analysis of ascorbic acid was carried out using an Agilent 1260 instrument with a VWD detector (Agilent Technologies, Inc., Palo Alto, USA). Samples were separated on a Waters Spherisorb C18 column (150 mm × 4.6 mm i.d.; 5 μm particle size), using a solvent of 0.1% oxalic acid at a flow rate of 1.0 mL min−1. The amount of ascorbic acid was calculated from absorbance values at 243 nm, using authentic ascorbic acid (Sangon Biotech Co., Ltd., shanghai, China) as a standard.
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