8 ohdg assay kit

Calcium oxalate monohydrate (COM) crystals were purchased from Macklin (Shanghai, China). COM crystals were weighed and suspended in sterile phosphate buffer solution at 1 mM and then were diluted into different concentrations. Ang II was purchased from Sangon Biotech (Shanghai, China). Anti-AT1R, anti-NADPH oxidase 2 (Nox2), anti-p50, and anti-p65 antibodies were purchased from Proteintech (Wuhan, China). Losartan and apocynin were purchased from Sigma Aldrich (St. Louis, MO, USA). 2′,7′-Dichlorofluorescein diacetate (DCF-DA) and Fluo-3AM were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Anti-NADPH oxidase 4 (Nox4), anti-OPN, and anti-MCP-1 antibodies were purchased from Abcam (Cambridge, MA, USA). An anti-CD44 antibody was purchased from Cell Signaling Technology (Boston, MA, USA). Malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China). An 8-OHdG assay kit was purchased from Elabscience (Wuhan, China). The transfection reagent Lipofectamine™ 2000 was purchased from Invitrogen (Carlsbad, CA, USA).

Tannic acid was purchased from Shanghai Macklin Biochemical Technology Co., Ltd., (Shanghai, China). MTT was obtained from Solarbio (Beijing, China). The bicinchoninic acid (BCA) protein assay kit, LDH cytotoxicity assay kit, superoxide dismutase (SOD) assay kit, catalase (CAT) assay kit, glutathione peroxidase (GSH-PX) assay kit, lipid peroxidation (MDA) assay kit, and ATP assay kit were purchased from Nanjing KeyGen Biotech. Co. Ltd (Nanjing, China). The Annexin V-FITC apoptosis detection kit and mitochondrial membrane potential assay kit were obtained from Nanjing KeyGen Biotech. Co. Ltd. The ROS content assay kit was purchased from Beyotime Biotech. Co. Ltd. (Nantong, China). Rabbit monoclonal antibody of Bcl-2, Bax, PARP-1, caspase-3, caspase-9, cytochrome c, P53, ATM, P-ATM. CDK, P-CDK, cyclin A2, and β-actin, and goat anti-rabbit antibody of IgG-HRP were obtained from Cell Signaling Technology (Boston, MA, USA). Z-LEHD-FMK was purchased from Abcam, Inc. (Cambridge, UK). The 8-OHdG assay kit was purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China).