L linalool

All chemicals used in this study were of analytical grade. PHB pellets (Average MW: 550,000 g/mol) were obtained from Goodfellow Cambridge Ltd. (Huntingdon, UK). Chloroform and ethanol were obtained from Fisher Scientific (Hampton, NH, USA). Bis(2-ethylhexyl) adipate, bis(2-ethylhexyl) sebacate, lauric acid, triacetin, tributyl citrate, tributyl 2-acetylcitrate, l-Linalool, and geraniol were obtained from Sigma-Aldrich (St. Louis, MO, USA).

The flavonoids aglycones luteolin, chrysoeriol and kaempferol were obtained from Extrasynthèse S.A. (Lyon, France). Vitexin-2-O-rhamnoside, apigenin-7-O-glucuronide, apigenin, quercetin, cannabigerolic acid (CBGA), tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA) were purchased from Sigma-Aldrich Chemie GmbH (Buchs, Switzerland) and luteolin-7-O-glucuronide from HWI-group (Rülzheim, Germany). (+)-alpha-Pinene, (−)-beta-Pinene, beta-Myrcene, Ocimene, L-Linalool, (E)-beta-Caryophyllene, alpha-Humulene, (−)-alpha-Bisabolol, S(−)-Limonene, alpha-Phellandrene, 1.8-Cineol, Terpinolene and gamma-Terpinene were purchased from Sigma-Aldrich Chemie GmbH (Buchs, Switzerland).
Acetonitrile (LC-MS grade) was supplied by VWR International GmbH (Dietikon, Switzerland). Water (LC-MS grade) and methanol (HPLC grade) were supplied by Carl Roth AG (Arlesheim, Switzerland). Trifluoroacetic acid (TFA) and dimethysulfoxide (DMSO) were supplied by Sigma-Aldrich Chemie GmbH (Buchs, Switzerland).

Fucoidan extracted from Fucus vesiculosus (92.9% fucoidan phytonutrient content, high molecular weight, code 5619002900, batch FVF2016507) was a kind gift from Kraeber & Co. GMBH (Ellerbek, Germany). Sodium alginate (from Lessonia nigrescens, code 177775000, lot A0376873, sulfated ash 34.16% (on dried substance)) was purchased from ACROS OrganicsTM (Thermo Fisher Scientific, Waltham, MA, USA). (−)-Menthol was acquired from Fluka (Buchs, Switzerland). Bergamot oil was purchased from Pranarôm (Ghislenghien, Belgium). L-Linalool, (−)-β-pinene, calcein, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) hemisodium salt, and trypsin (from the porcine pancreas) were acquired from Sigma-Aldrich (St. Louis, MO, USA). The porcine ears were purchased at a local slaughter (Porto, Portugal). Double-deionized water was provided by an ultra-pure water system (Arium Pro, Sartorius AG, Gottingen, Germany). The reagents were weighted in a digital analytical balance Kern ACJ/ACS 80-4 (Kern & Sohn; Balingen, Germany). All other reagents were analytical grade and were used without any further purification.