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2 protocols using alloxan monohydrate

1

Antioxidant and Enzyme Inhibition Assays

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The following drugs and solvents were used in this study: 2, 2-diphenyl-1-picrylhydrazyl [DPPH] (Alfa Aesar, Hamburg, Germany), L-ascorbic acid (Sigma Aldrich, Dorset, UK), linoleic acid (Sigma Aldrich, St. Louis, MO, USA), Tween 40 (Sigma Aldrich, St. Louis, MO, USA), β-carotene (Sigma Aldrich, St. Louis, MO, USA), Potassium ferricyanide K3[Fe(CN)6] (Sigma Aldrich, St. Louis, MO, USA),Trichloroacetic acid (TCA) (Sigma Aldrich, St. Louis, MO, USA), Ferric chloride FeCl3 (Sigma Aldrich, St. Louis, MO, USA), Alloxan monohydrate (Allx monohydrate 98%, ACROS Organics), Streptozotocin (Sigma-Aldrich, Hamburg, Germany), α-amylase and acarbose (Sigma Aldrich, St. Louis, MO, USA), glucose oxidase–peroxidase (GOD–POD) (Biosystems, Barcelona, Spain), starch (Sigma Aldrich, Riedel-de Haen, Germany), Folin–Ciocalteu, gallic acid, aluminum chloride, and quercetin (Sigma Aldrich, St. Louis, MO, USA). The solvents utilized were chloroform (Sigma Aldrich, Riedel-de Haen, Germany), diethyl ether (Sigma Aldrich, Riedel-de Haen, Germany), and methanol (Sigma Aldrich, Riedel-de Haen, Germany). All chemicals and solvents used were analytical grades.
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2

Antioxidant and Antidiabetic Potential Assay

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The following reagents were purchased from Sig-maAldrich: The starch and the sucrose powders, the αglucosidase enzyme, the α-amylase enzyme, dinitrosalicylic acid, Acarbose, phlorizin dehydrate, D-glucose anhydrous, potassium chloride (KCl), magnesium chloride-6-hydrate (MgCl 2 ꞏ6H 2 O), sodium chloride (NaCl), folinciocalteu, gallic acid, ascorbic acid, quercetin, DPPH, aluminum chloride (AlCl 3 ), potassium ferricyanide [K 3 Fe (CN) 6 ], methanol, ethanol, chloroform, and ferric chloride (FeCl 3 ), Trichloroacetic acid (TCA). Calcium chloride dihydrate (CaCl 2 ꞏ2H 2 O) purchased from ScharlauChemie S.A., Spain. Sodium hydrogen carbonate (NaHCO 3 ) were purchased from Farco Chemical Supplies, (Puerto Rico). Sodium phosphate monobasic 2-hydrate NaH 2 PO 4 ꞏ2H 2 O purchased from Panreac, Spain. Alloxan mono-hydrate was purchased from ACROS Organics. Pentobarbital obtained from CEVA Santé Animale, France. Ether obtained from Somaprol, Casablanca, Morocco.
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