Protein was harvested from approximately 300 mg of flash-frozen tissue homogenized in RIPA buffer (25 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, pH 7.5) with Complete mini proteinase inhibitors (Roche). A total of 20−30 μg of protein was separated on 10% Novex Tris-Glycine gels (Invitrogen, Thermo Fisher Scientific) and transferred to PVDF membranes. Total protein stain (30% methanol, 6.7% acetic acid, 0.0005% Fast Green FCF from Merck KGaA) stain was used as a loading control and imaged prior to blocking. After blocking, human SMN protein was probed for using anti-SMN, clone SMN-KH monoclonal IgG1 (Merck Millipore, MABE230), and secondary antibody IRDye 800CW goat anti-mouse IgG (LI-COR Biosciences, 926-32210). Membranes were imaged on Odyssey FC imager and analyzed with Image Studio software (both LI-COR Biosciences).
Smn kh monoclonal igg1
Manufactured by Merck Group
The SMN-KH monoclonal IgG1 is a laboratory research product manufactured by Merck Group. It is a monoclonal antibody of the IgG1 isotype. The core function of this product is to serve as a tool for research and scientific investigations, though its specific intended uses are not provided.
Automatically generated - may contain errors
Lab products found in correlation
Irdye 800cw goat anti mouse igg, by LI COR (2 mentions)
Novex tris glycine gel, by Thermo Fisher Scientific (2 mentions)
Complete mini proteinase inhibitor, by Roche (2 mentions)
Image studio software, by LI COR (2 mentions)
Fast green fcf, by Merck Group (2 mentions)
Odyssey fc imager, by LI COR (2 mentions)
2 protocols using smn kh monoclonal igg1
1
Quantifying SMN Protein from Tissue
2
Quantifying Protein Expression in Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein was harvested from approximately 300 mg of flash-frozen tissue homogenized in RIPA buffer (25 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, pH 7.5) with Complete mini proteinase inhibitors (Roche). A total of 20–30 μg of protein was separated on 10% Novex Tris-Glycine gels (Invitrogen, Thermo Fisher Scientific) and transferred to PVDF membranes. Total protein stain (30% methanol, 6.7% acetic acid, 0.0005% Fast Green FCF from Merck KGaA) stain was used as a loading control and imaged prior to blocking. After blocking, human SMN protein was probed for using anti-SMN, clone SMN-KH monoclonal IgG1 (Merck Millipore, MABE230), and secondary antibody IRDye 800CW goat anti-mouse IgG (LI-COR Biosciences, 926-32210). Membranes were imaged on Odyssey FC imager and analyzed with Image Studio software (both LI-COR Biosciences).
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