Log In Sign up
The largest database of trusted experimental protocols

Anti α smooth muscle actin clone 1a4

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Anti-α-smooth muscle actin (clone 1A4) is a monoclonal antibody used for the identification and localization of α-smooth muscle actin in various cell types and tissues. It is a reliable marker for smooth muscle cells and myofibroblasts.

Automatically generated - may contain errors

Lab products found in correlation

Anti collagen 4, by Abcam (1 mentions) Anti involucrin clone sy5, by Merck Group (1 mentions) Anti collagen 1, by Abcam (1 mentions) Anti vimentin clone v9, by Agilent Technologies (1 mentions) Alexa 488 anti mouse igg, by Thermo Fisher Scientific (1 mentions) Anti thy1 clone 5e10, by BD (1 mentions) Anti fibronectin, by Abcam (1 mentions) Anti phospho smad1 5 8, by Cell Signaling Technology (1 mentions) Recombinant human bmp6, by R&D Systems (1 mentions) Anti hif 2α nb100 122, by Novus Biologicals (1 mentions) Mg132, by Peptide Institute (1 mentions) Anti hif 1α nb100 449, by Novus Biologicals (1 mentions) Anti flag m2 f3165, by Merck Group (1 mentions) Basic fibroblast growth factor (bfgf), by R&D Systems (1 mentions) Vascular endothelial growth factor (vegf), by R&D Systems (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Tgf β, by R&D Systems (1 mentions)

Close spelling variants of this product

Mouse anti-α-smooth muscle actin (α-SMA) (clone 1A4, Anti-α-smooth muscle actin mouse monoclonal antibody (clone 1A4) α-smooth muscle actin (clone 1A4 Smooth muscle actin (clone 1A4, Anti-α-smooth muscle actin α-smooth muscle actin Clone 1A4 Anti-α-actin α-actin Anti-actin

2 protocols using anti α smooth muscle actin clone 1a4

1

Antibody Characterization for Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary rabbit polyclonal antibodies used were: anti-collagen I (Abcam; Cambridge, UK), anti-collagen IV (Abcam), anti-fibronectin (Abcam) and the anti-perlecan antibody CCN-1 was a gift from Prof. John Whitelock (University of NSW, Sydney, Australia). The mouse monoclonal antibodies (mAbs) used were: anti-fibroblast marker (clone TE7; Millipore; MA, USA), anti-involucrin (clone SY5; Sigma; MO, USA), anti-ki67 (clone MM1; Novacastra; Wetzlar, Germant), anti-p63 (clone 4A4; Abcam), anti-α-smooth muscle actin (clone 1A4; Sigma), anti-Thy1 (clone 5E10, BD Bioscience; NJ, USA) and anti-vimentin (clone V9; Dako; CA, USA). The mouse mAbs anti-keratin 14 (K14, clone LL001), anti-keratin 16 (K16, clone LL025) and anti-keratin 10 (K10, clone LH2) were produced in the labs of EBL and IM Leigh71 (link),72 (link). The secondary antibodies used were Alexa488 anti-mouse IgG, Alexa546 anti-mouse IgG, Alexa 488 anti-rabbit IgG and Alexa546 anti-rabbit IgG (all from Molecular Probes, ThermoFisher Scientific; OR, USA).
Wong C.W., LeGrand C.F., Kinnear B.F., Sobota R.M., Ramalingam R., Dye D.E., Raghunath M., Lane E.B, & Coombe D.R. (2019). In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics. Scientific Reports, 9, 18561.
+ Open protocol
+ Expand
2

Antibody-Based Analysis of BMP and VEGF Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human BMP-6, BMP-9, VEGF, bFGF and TGF-β were
purchased from R&D Systems (R&D Systems, Bio-Techne, Minneapolis,
MN, USA). Cycloheximide (CHX) was purchased from Sigma-Aldrich (Merck,
Darmstadt, Germany), and MG132 was from Peptide Institute (Osaka, Japan). The
following antibodies were used: anti-FLAG M2 (F3165; Sigma-Aldrich, Merck),
anti-DDDDK (PM020; MBL, Tokyo, Japan) which recognizes the FLAG epitope,
anti-Myc, anti-HA (HA124; Nacalai Tesque, Kyoto, Japan), anti-HA (#561; MBL),
anti-α-tubulin (AC-15; Sigma-Aldrich, Merck), anti-SMAD1 (913C1b; Bio
Matrix Research, Chiba, Japan) which recognizes both SMAD1 and SMAD5 for ChIP
assays (5 (link)), anti-phospho-SMAD1/5/8 (Cell
Signaling Technology, Danvers, MA, USA), anti-PECAM1 (clone 18; BD bioscience,
San Jose, CA, USA), anti-HIF-1α (NB100-449, Novus Biologicals,
Bio-Techne), anti-HIF-2α (NB100-122, Novus Biologicals, Bio-Techne),
anti-α-smooth muscle actin (clone 1A4; Sigma-Aldrich, Merck), and
anti-HDAC1 (clone 2E10, #05-100; Millipore, Merck). A rabbit polyclonal
anti-ATOH8 antibody was raised against residues 306-321 of human ATOH8,
CTRTLQAEGRAKKRKE.
Morikawa M., Mitani Y., Holmborn K., Kato T., Koinuma D., Maruyama J., Vasilaki E., Sawada H., Kobayashi M., Ozawa T., Morishita Y., Bessho Y., Maeda S., Ledin J., Aburatani H., Kageyama R., Maruyama K., Heldin C.H, & Miyazono K. (2019). The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Science signaling, 12(607), eaay4430.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!