Log In Sign up
The largest database of trusted experimental protocols

Neutral insulin

Manufactured by Novo Nordisk
Visit Supplier
Sourced in Denmark

Neutral Insulin is a laboratory product used for research and development purposes. It serves as a baseline insulin compound for further analysis and development. The product provides a standardized insulin formulation to support scientific investigations and experiments.

Automatically generated - may contain errors

Lab products found in correlation

Citrate buffer, by Merck Group (2 mentions) Streptozotocin (stz), by Merck Group (2 mentions) Freestyle freedom, by Abbott (1 mentions)

Close spelling variants of this product

Insulin

3 protocols using neutral insulin

1

Streptozotocin-Induced Diabetic Rat Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Male Sprague-Dawley (SD, 8 weeks old) rats were obtained from Jackson Research Laboratories (Bar Harbor, ME). Care, use, and treatment of all animals in this study were approved by the Animal Care Committee at Icahn School of Medicine at Mount Sinai. Diabetes was induced in SD rats (8 weeks of age) by an intraperitoneal injection of streptozotocin (STZ) (60mg/kg in 10mmol/L of citrate buffer, pH 4.5) (Sigma, St. Louis, MO) after an overnight fast. Control rats received an injection of citrate buffer. Blood glucose levels were measured at 48 hours after the injection and monitored every 3 days thereafter. Only the animals with blood glucose concentrations >16.7mmol/l were considered diabetic. A group of diabetic rats received Neutral Insulin (16U/kg; Novo Nordisk, Copenhagen, Denmark) via subcutaneous injection twice a day from 6 to 12 weeks of diabetes for hyperglycemic control. Non-fasting glucose was monitored before the insulin injection. The kidney was removed when the mice were sacrificed for glomerular isolation and histology. 12-hour-urine samples were collected on the day before euthanasia by housing the animals in metabolic cages to determine the urine albumin excretion rate (Houston, TX).
Zhong F., Chen H., Wei C., Zhang W., Li Z., Jain M.K., Chuang P.Y., Chen H., Wang Y., Mallipattu S.K, & He J.C. (2014). Reduced Krüppel-like Factor 2 expression may aggravate the endothelial injury of diabetic nephropathy. Kidney international, 87(2), 382-395.
+ Open protocol
+ Expand
2

Glucose and Insulin Tolerance in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
After a 1-week acclimation, male C57BL/6J mice were fasted for 10 h before the intraperitoneal glucose tolerance test (GTT) at week 19. After a sample of fasted blood was collected from tail bleeding, animals were given glucose (1 g/kg body weight) by intraperitoneal injection. Blood glucose readings were taken using a glucometer (Freestyle Freedom, Abbott Laboratories) at 0, 30, 60, and 120 min after injection. Insulin tolerance tests (ITT) were carried out 1 week after GTTs. Neutral insulin (1 U/kg body weight, Novo Nordisk, Denmark) was injected intraperitoneally after a 6-hour fast. Blood glucose levels were measured at 0, 15, 30, 60, 90, and 120 min after injection. The areas under the curves for blood glucose in GTT (AUCg) and ITT (AUCitt) were calculated.
Zhu Y.X., Zhang M.L., Zhong Y., Wang C, & Jia W.P. (2015). Modulation Effect of Peroxisome Proliferator-Activated Receptor Agonists on Lipid Droplet Proteins in Liver. Journal of Diabetes Research, 2016, 8315454.
+ Open protocol
+ Expand
3

Streptozotocin-Induced Diabetic Rat Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Male Sprague-Dawley (SD, 8 weeks old) rats were obtained from Jackson Research Laboratories (Bar Harbor, ME). Care, use, and treatment of all animals in this study were approved by the Animal Care Committee at Icahn School of Medicine at Mount Sinai. Diabetes was induced in SD rats (8 weeks of age) by an intraperitoneal injection of streptozotocin (STZ) (60mg/kg in 10mmol/L of citrate buffer, pH 4.5) (Sigma, St. Louis, MO) after an overnight fast. Control rats received an injection of citrate buffer. Blood glucose levels were measured at 48 hours after the injection and monitored every 3 days thereafter. Only the animals with blood glucose concentrations >16.7mmol/l were considered diabetic. A group of diabetic rats received Neutral Insulin (16U/kg; Novo Nordisk, Copenhagen, Denmark) via subcutaneous injection twice a day from 6 to 12 weeks of diabetes for hyperglycemic control. Non-fasting glucose was monitored before the insulin injection. The kidney was removed when the mice were sacrificed for glomerular isolation and histology. 12-hour-urine samples were collected on the day before euthanasia by housing the animals in metabolic cages to determine the urine albumin excretion rate (Houston, TX).
Zhong F., Chen H., Wei C., Zhang W., Li Z., Jain M.K., Chuang P.Y., Chen H., Wang Y., Mallipattu S.K, & He J.C. (2014). Reduced Krüppel-like Factor 2 expression may aggravate the endothelial injury of diabetic nephropathy. Kidney international, 87(2), 382-395.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!