The following antibodies were used in this study: anti-B7-H3 (FM276, Miltenyi Biotech), anti-GD2 (14.G2a, BD Biosciences), human Ig (polyclonal, Thermo Fisher Scientific), anti-mouse IgG (polyclonal, R&D), anti-CD3 (UCHT1, BioLegend), anti-HisTag (J095G45, BioLegend), anti-CD34 (QBEnd10, R&D), anti-ab-TCR (IP26, BioLegend), anti-CD107a (H4A3, BioLegend), anti-cD25 (BC96, BioLegend), anti-CD69 (FN50, BioLegend), anti-Tim3 (F38-2E2, BioLegend), anti-Lag3 (11C3C65, BioLegend), anti-PD-1 (EH12.1, BD Biosciences), anti-mouse CD45 (30-F11, BioLegend), anti-human CD45 (HI30, BioLegend), Ghost Red 780 (Tonbo Biosciences), Zombie Yellow Viability Dye (BioLegend), propidium iodide (Gibco), Cell Trace Violet (Thermo Fisher Scientific), and Precision Count Beads (BioLegend).
Anti pd 1 eh12
Manufactured by BD
Sourced in United States
The Anti-PD-1 (EH12.1) is a monoclonal antibody targeting the programmed cell death protein 1 (PD-1). It is used as a research tool for studying the PD-1 receptor and its role in immune regulation.
Automatically generated - may contain errors
Lab products found in correlation
Anti tim 3 f38 2e2, by BioLegend (3 mentions)
Anti lag 3 11c3c65, by BioLegend (3 mentions)
Permeabilization buffer, by Thermo Fisher Scientific (2 mentions)
Anti tigit mbsa43, by Thermo Fisher Scientific (2 mentions)
Anti ifn γ 4s b3, by Thermo Fisher Scientific (2 mentions)
Il 10 jes3 9d7, by BioLegend (2 mentions)
Cytofix fixation buffer, by BD (2 mentions)
Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
Ionomycin, by Merck Group (2 mentions)
Phorbol 12 myristate 13 acetate, by Merck Group (2 mentions)
Brefeldin a, by BD (2 mentions)
Celltrace violet, by Thermo Fisher Scientific (2 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
Precision count beads, by BioLegend (1 mentions)
Zombie yellow viability dye, by BioLegend (1 mentions)
Ghost red 780, by Cytek Biosciences (1 mentions)
Anti cd3 ucht1, by BioLegend (1 mentions)
Anti cd69 fn50, by BioLegend (1 mentions)
Anti cd25 bc96, by BioLegend (1 mentions)
Anti mouse cd45 30 f11, by BioLegend (1 mentions)
4 protocols using anti pd 1 eh12
1
Comprehensive Immunological Antibody Panel
2
Multiparameter Immune Profiling of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were stained with LIVE/DEAD Fixable Near-IR Dead Cell Stain kit (Invitrogen) and surface antibodies for 30 min at 4 °C. For intracellular cytokine staining, cells were treated with 50 nM phorbol-12-myristate-13-acetate (MilliporeSigma) and 250 nM ionomycin (MilliporeSigma) for 4 hours in the presence of Brefeldin A (BD Biosciences) before harvesting. Cells were washed and fixed with BD Cytofix™ Fixation Buffer (BD Biosciences) for 10 min at RT, then washed with PBS. Intracellular cytokines were stained in permeabilization buffer (eBioscience) for 30 min at 4 °C. The following antibodies were used: anti-LAG-3 (11C3C65, BioLegend), anti-PD-1 (EH12.1, BD Biosciences), anti-TIGIT (MBSA43, eBioscience), anti-Tim-3 (F38–2E2, Biolegend), anti-IFN-γ (4S.B3, eBioscience), and IL-10 (JES3–9D7, Biolegend). Cells were acquired on a BD Fortessa flow cytometer and data was analyzed with FlowJo software v10 (Threestar).
3
Phenotypic Profiling of Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were stained with LIVE/DEAD Fixable Near-IR Dead Cell Stain kit (Invitrogen) and surface antibodies for 30 min at 4 °C. For intracellular cytokine staining, cells were treated with 50 nM phorbol-12-myristate-13-acetate (MilliporeSigma) and 250 nM ionomycin (MilliporeSigma) for 4 hours in the presence of Brefeldin A (BD Biosciences) before harvesting. Cells were washed and fixed with BD Cytofix™ Fixation Buffer (BD Biosciences) for 10 min at RT, then washed with PBS. Intracellular cytokines were stained in permeabilization buffer (eBioscience) for 30 min at 4 °C. The following antibodies were used: anti-LAG-3 (11C3C65, BioLegend), anti-PD-1 (EH12.1, BD Biosciences), anti-TIGIT (MBSA43, eBioscience), anti-Tim-3 (F38-2E2, Biolegend), anti-IFN-γ (4S.B3, eBioscience), and IL-10 (JES3-9D7, Biolegend). Cells were acquired on a BD Fortessa flow cytometer and data was analyzed with FlowJo software v10 (Threestar).
4
Isolation and Analysis of Tumor-Infiltrating Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumors were excised from mice and minced finely using a scalpel blade. Minced tumors were placed in 5 ml of RPMI 1640:DMEM media (1:1), containing collagenase type 4 (1 mg/ml, Sigma St Louis, MO, USA) and DNase I (20 μg/ml, Roche, Basel, Switzerland). Tumors were digested at 37 °C for 45 min, rotating and rolling gently using a MACSmix Tube rotator (Miltenyi Biotec, Germany). Tumor suspensions were then passed through a 40-μm cell strainer and washed twice in RPMI 1640 complete media. Isolated TILs were then stained with anti-CD45 (30-F11), anti-CD11b (M1/70), anti-CD8 (53-6.7), anti-CD4 (RM4-5), anti-NK1.1 (PK136), anti-PD1 (EH12.1) and anti-TIM3 (5D12) (all BD Pharmingen, San Diego, CA, USA). TILs were analyzed by flow cytometry using a FACS Canto instrument or Fortessa (BD Biosciences, Franklin Lakes, NJ, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!