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7500 fast system sds software version 1

Manufactured by Thermo Fisher Scientific
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The 7500 Fast System SDS software version 1.3.1 is a software application designed to operate the 7500 Fast Real-Time PCR System, a laboratory instrument used for nucleic acid amplification and detection. The software version 1.3.1 provides the necessary functionality to control the instrument, set up and manage experiments, and analyze the resulting data.

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Lab products found in correlation

Prism 7500 fast sequence detection system, by Thermo Fisher Scientific (5 mentions) Taqman universal pcr master mix, by Thermo Fisher Scientific (5 mentions) Iscript cdna synthesis kit, by Bio-Rad (3 mentions) Rneasy mini kit, by Qiagen (3 mentions) Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Tri reagent, by Molecular Research Center (1 mentions) Gapdh hs02758991 g1, by Thermo Fisher Scientific (1 mentions) Human apoptosis rt2 profiler pcr array, by Qiagen (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Iscript synthesis kit, by Bio-Rad (1 mentions) Taqman probes and primers, by Thermo Fisher Scientific (1 mentions) Taqman fast universal pcr master mix, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

7500 Fast System SDS Software 1.3.1 7500 System SDS software version 1.3.1 7500 Fast System SDS software 7500 Fast System software 1.4 7500 fast system SDS System SDS software version 1.2.3 7500 System SDS software 7500 Fast System Software SDS software version 1.3.1 7500 SDS software

6 protocols using 7500 fast system sds software version 1

1

Quantitative Analysis of MLH1 Expression

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Extracted RNA was reverse-transcribed into complementary DNA (cDNA) using iScript cDNA Synthesis kit (Bio-Rad) and TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems, Foster City, CA). Quantitative real-time PCR analysis was performed with an Applied Biosystems Prism 7500 Fast Sequence Detection System using TaqMan Universal PCR master mix according to the manufacturer's protocol (Applied Biosystems). Levels of RNA expression were determined using the 7500 Fast System SDS software version 1.3.1 (Applied Biosystems). PCR parameters for cycling were as follows: 95°C for 20 seconds, then 40 cycles of 95°C for 3 seconds and 60°C for 30 seconds. All reactions were done in a 10 μL reaction volume in triplicate. The data were analyzed using the delta-delta Ct method to calculate the fold-change. TaqMan probes and primers for MLH1 (assay ID: Hs00179866_m1) and GAPDH (assay ID: Hs02758991_g1) were obtained from Applied Biosystems. GAPDH was used as internal control.
Fukuhara S., Chang I., Mitsui Y., Chiyomaru T., Yamamura S., Majid S., Saini S., Hirata H., Deng G., Gill A., Wong D.K., Shiina H., Nonomura N., Dahiya R, & Tanaka Y. (2014). DNA mismatch repair gene MLH1 induces apoptosis in prostate cancer cells. Oncotarget, 5(22), 11297-11307.
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2

Osteoblast gene expression analysis

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Total RNA was extracted from osteoblasts using the RNeasy mini kit (Qiagen) following the manufacturer’s instruction. RNA concentration was measured by the NanoDrop spectrophotometer. Synthesis of cDNA was performed using 1 µg of total RNA and the High‐Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific), following the manufacturer’s instructions. Comparative real-time PCR was performed in triplicate with an Applied Biosystems Prism 7500 Fast Sequence Detection System using TaqMan universal PCR master mix, according to the manufacture’s protocol (Applied Biosystems Inc., Foster City, CA) (Fig. 5a, f, g). The TaqMan probes and primers were purchased from Applied Biosystems: Human RUNX2 (Hs01047973_m1), COL1A1 (Hs00164004_m1), ALPL (Hs01029144_m1), RANKL (TNFSF11, Hs00243522_m1), OPG (TNFRSF11B, Hs00900358_m1). Human GAPDH (Hs02786624_g1) and TBP (Hs00427620_m1) were used as endogenous controls for normalization. Levels of MAP2K1, RUNX2, ALPL and COL1A1, RANKL (TNFSF11), and OPG (TNFRSF11B) transcripts were determined using the 7500 Fast System SDS software version 1.3.1 (Applied Biosystems). Relative expression was calculated using the comparative ∆∆Ct method.
Kang H., Jha S., Deng Z., Fratzl-Zelman N., Cabral W.A., Ivovic A., Meylan F., Hanson E.P., Lange E., Katz J., Roschger P., Klaushofer K., Cowen E.W., Siegel R.M., Marini J.C, & Bhattacharyya T. (2018). Somatic activating mutations in MAP2K1 cause melorheostosis. Nature Communications, 9, 1390.
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3

Quantitative Gene Expression Analysis

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Total RNA from clinical samples and cell lines were extracted using TRI reagent (Molecular Research Center, Cincinnati, OH) and RNeasy Mini kit (Qiagen, Valencia, CA), respectively. Extracted RNA was reverse-transcribed into cDNA using iScript cDNA Synthesis kit (Bio-Rad). Quantitative real-time PCR analysis was performed with an Applied Biosystems (Foster City, CA) Prism 7500 Fast Sequence Detection System using TaqMan Universal PCR master mix, probes and primers for target genes. The mRNA transcript levels of target genes were determined using the 7500 Fast System SDS software version 1.3.1 (Applied Biosystems). For clinical samples, a standard curve was generated using a serial dilution of external standards. The level of expression was calculated as the ratio of the target gene to that of the reference glyceraldehyde-3-phosphate dehydrogenase (GAPDH). For cell lines, the data were analyzed by the delta-delta Ct method to calculate fold-change.
Mitsui Y., Shiina H., Kato T., Maekawa S., Hashimoto Y., Shiina M., Imai-Sumida M., Kulkarni P., Dasgupta P., Wong R.K., Hiraki M., Arichi N., Fukuhara S., Yamamura S., Majid S., Saini S., Deng G., Dahiya R., Nakajima K, & Tanaka Y. (2017). Versican Promotes Tumor Progression, Metastasis and Predicts Poor Prognosis in Renal Carcinoma. Molecular cancer research : MCR, 15(7), 884-895.
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4

Quantitative Analysis of Apoptosis Genes

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Total RNA was extracted from DU145, PWR-1E and RWPE-1 cells using the RNeasy Mini kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. Extracted RNA was reverse-transcribed into complementary DNA (cDNA) using iScript cDNA Synthesis kit (Bio-Rad) and TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems, Foster City, CA). Quantitative real-time PCR analysis was performed with an Applied Biosystems Prism 7500 Fast Sequence Detection System using TaqMan Universal PCR master mix according to the manufacturer's protocol (Applied Biosystems). Levels of RNA expression were determined using the 7500 Fast System SDS software version 1.3.1 (Applied Biosystems). PCR parameters for cycling were as follows: 95ºC for 20 seconds, then 40 cycles of 95ºC for 3 seconds and 60ºC for 30 seconds. All reactions were done in a 10 μL reaction volume in triplicate. The data were analyzed using the delta-delta Ct method to calculate the fold-change. TaqMan probes and primers for PMS2 (assay ID: Hs00241053_m1), TMS1 (Hs01547324_gH), BCL2A1 (Hs00187845_m1), and GAPDH (Hs02758991_g1) were obtained from Applied Biosystems. GAPDH was used as internal control. For array analyses, expression of apoptosis-related genes was determined using the Human Apoptosis RT2 Profiler PCR Array (Qiagen) per manufacturer's instructions.
Fukuhara S., Chang I., Mitsui Y., Chiyomaru T., Yamamura S., Majid S., Saini S., Deng G., Gill A., Wong D.K., Shiina H., Nonomura N., Lau Y.F., Dahiya R, & Tanaka Y. (2015). Functional role of DNA mismatch repair gene PMS2 in prostate cancer cells. Oncotarget, 6(18), 16341-16351.
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5

Quantitative Real-Time RT-PCR Analysis

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Quantitative real-time RT-PCR was performed in triplicate with an Applied Biosystems Prism 7500 Fast Sequence Detection System using TaqMan universal PCR master mix according to the manufacture’s protocol (Applied Biosystems Inc., Foster City, CA, USA). The TaqMan probes and primers were purchased from Applied Biosystems. Human GAPDH and RNU48 were used as endogenous controls. Levels of RNA expression were determined using the 7500 Fast System SDS software version 1.3.1 (Applied Biosystems).
Hirata H., Hinoda Y., Shahryari V., Deng G., Nakajima K., Tabatabai Z.L., Ishii N, & Dahiya R. (2015). Long noncoding RNA MALAT1 promotes aggressive renal cell carcinoma through Ezh2 and interacts with miR-205. Cancer research, 75(7), 1322-1331.
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6

Quantitative Real-Time PCR Analysis

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cDNA was synthesized using an iScript Synthesis Kit (Bio-Rad). Quantitative real-time PCR (qPCR) analysis was performed in triplicate with an Applied Biosystems Prism 7500 fast Sequence Detection System using TaqMan Fast Universal PCR Master Mix according to the manufacturer’s protocol (Applied Biosystems). The PCR program was as follows: denaturation at 95 °C for 15 min and 45 cycles of amplification consisting of denaturation at 95 °C for 15 s, annealing at 58 °C for 30 s, and extension at 72 °C for 30 s. TaqMan probes and primers were purchased from Applied Biosystems. Human GAPDH were used as an internal control. Levels of RNA expression were determined by using the 7500 Fast System SDS software version 1.3.1 (Applied Biosystems).
Imai-Sumida M., Dasgupta P., Kulkarni P., Shiina M., Hashimoto Y., Shahryari V., Majid S., Tanaka Y., Dahiya R, & Yamamura S. (2020). Genistein represses HOTAIR/chromatin remodeling pathways to suppress kidney cancer. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 54(1), 53-70.
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