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Diprotin

Manufactured by Merck Group
Sourced in United States

Diprotin is a laboratory instrument used for the analysis and quantification of dipeptidyl peptidase-IV (DPP-IV) enzyme activity. It measures the enzymatic cleavage of a specific dipeptide substrate, providing information about DPP-IV levels in biological samples.

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2 protocols using diprotin

1

Metabolic Regulation by Bioactive Compounds

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Resveratrol (CHEM-IMPEX INT’L INC), taxifolin (Sigma aldrich), acarbose
(Carbosnyth,USA), diprotin (Sigma aldrich), HPA assay kit (Product code: K-CERA,
Megazyme brand), α-glucosidase assay kit (Product code: MAK123, Sigma aldrich),
DPP-IV inhibitor screening assay kit (Product code: ab133081 Abcam), starch
(Sigma aldrich) and all the other materials of analytical grade were used.
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2

AMPK Activation Pathway Modulation

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Diprotin was from Sigma Chemical. Control lentiviral activation particles, AMPKα1 lentiviral activation particles, Polybrene, control siRNA, AMPKα1 siRNA and mouse anti-AMPKα1 antibody were purchased from Santa Cruz Inc (Santa Cruz, USA). AICAR, mouse antiβ-actin, rabbit anti-AMPKα (Thr172) and rabbit anti-AMPKα antibodies were obtained from Cell Signaling Technology. Rabbit anti-AMPKα2 antibody and mouse anti-GLP1 were from Abcam. IRDye-conjugated affinitypurified anti-rabbit, anti-mouse IgGs were purchased from Rockland (Gilbertsville, PA, USA). TRIzol reagent and the reverse transcription (RT) system were from Promega. Lipofectamine was purchased from Invitrogen. Compound C and Glucagon-Like Peptide-1 Active enzyme immunoassay kit were purchased from Millipore.
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