To construct pCMV6-XL5-ERBB1 which expresses human ERBB1, the full length human ERBB1 coding sequence was amplified by PCR from LNCaP cDNA using Kpn1-forward primer (5′-GGTACCCGGCCCCCTGACTCCGTCCAG-3′) and HindIII-reverse primer (5′-AAGCTTTCATGCTCCAATAAATTCACTGCTTTGTGGC-3′). The amplified PCR product was digested by KpnI and HindIII (New England BioLabs), followed by ligation into pCMV6-XL5 (Origene) which was pre-digested with the same restriction enzymes. The construct was sequenced to ensure the integrity of the entire coding sequence and correct orientation of the gene. We obtained human ERBB3-expressing plasmid (pCMV6-XL4-ERBB3) and human SRC-expressing plasmid (pCMV6-XL4-SRC) from Origene. Transient gene transfection was carried out using cells grown in 6-well plates and FuGENE HD (Promega).
Pcmv6 xl4 src
Manufactured by OriGene
The PCMV6-XL4-SRC is a plasmid that contains the coding sequence for the Src proto-oncogene. This plasmid can be used for expression of the Src protein in various cell lines.
Automatically generated - may contain errors
2 protocols using pcmv6 xl4 src
1
Constructing ERBB1 Expression Plasmid
2
Constructing ERBB1 Expression Plasmid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To construct pCMV6-XL5-ERBB1 which expresses human ERBB1, the full length human ERBB1 coding sequence was amplified by PCR from LNCaP cDNA using Kpn1-forward primer (5′-GGTACCCGGCCCCCTGACTCCGTCCAG-3′) and HindIII-reverse primer (5′-AAGCTTTCATGCTCCAATAAATTCACTGCTTTGTGGC-3′). The amplified PCR product was digested by KpnI and HindIII (New England BioLabs), followed by ligation into pCMV6-XL5 (Origene) which was pre-digested with the same restriction enzymes. The construct was sequenced to ensure the integrity of the entire coding sequence and correct orientation of the gene. We obtained human ERBB3-expressing plasmid (pCMV6-XL4-ERBB3) and human SRC-expressing plasmid (pCMV6-XL4-SRC) from Origene. Transient gene transfection was carried out using cells grown in 6-well plates and FuGENE HD (Promega).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!