Anti cpt 1 antibody

Manufactured by Abcam

Sourced in United States

The Anti-CPT-1 antibody is a laboratory reagent used in research applications. It is designed to specifically bind and detect the Carnitine Palmitoyltransferase 1 (CPT-1) protein, which is a key enzyme involved in the process of fatty acid oxidation. This antibody can be utilized in various experimental techniques, such as Western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of the CPT-1 protein in biological samples.

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Lab products found in correlation

Tetracycline, by Avantor (1 mentions) Anti cd36 antibody, by Abcam (1 mentions) Rosiglitazone rosi, by GlaxoSmithKline (1 mentions) Anti fas antibody, by Cell Signaling Technology (1 mentions) Anti acc antibody, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Cell Signaling Technology (1 mentions) Anti cd16 32, by BioLegend (1 mentions) Mayer s hematoxylin, by Agilent Technologies (1 mentions) Liquid dab substrate chromogen system, by Agilent Technologies (1 mentions) Eclipse ci microscope, by Nikon (1 mentions)

2 protocols using anti cpt 1 antibody

Lipid Metabolism Regulation Protocol

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Fenofibrate (Feno) from Fournier Pharma (France); Fluvastatin (Flu) from Beijing Novartis Pharma Ltd; Tetracycline was purchased from AMRESCO (USA); Polyene Phosphatidylcholine (PPC) from Sanofi, Beijing (China); Rosiglitazone (Rosi) from GSK, China; Anti-β-actin antibody, anti-FAS antibody, anti-ACC antibody from Cell Signaling Technology (USA); anti-MTTP antibody, anti-CPT-1 antibody, anti-CD36 antibody from Abcam (USA); anti-SREBP-1c antibody from SANTA (USA); commercial kits for liver triglyceride, ECL reactions from Applygen Technologies Inc.

Yi Q., Sun P., Li J., Kong S., Tian J., Li X., Yang Y., Zhang P., Liu Y., Han J., Zhang X, & Ye F. (2018). Rho, a Fraction From Rhodiola crenulate, Ameliorates Hepatic Steatosis in Mice Models. Frontiers in Physiology, 9, 222.

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Immunohistochemical Analysis of CPT1 in Mice

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Serial tissue sections from each mouse, 5 μm in thickness, were deparaffinized, rehydrated, and processed for antigen retrieval in citrate buffer using a vegetable steamer. Sections were blocked with a TBS solution containing Tween-20, BSA, goat serum, and anti-CD16/32 (Biolegend) and incubated with an anti-CPT1 antibody (Abcam). Sections were treated with hydrogen peroxide prior to incubation with an HRP labelled polymer conjugated to goat anti-rabbit immunoglobulins (EnVision+, Dako). HRP was detected using the Liquid DAB+ Substrate Chromogen system (Dako). Mayer’s hematoxylin (Dako) was used to counterstain. Three sections from each mouse were stained and then imaged using a Nikon Eclipse Ci microscope.  Analysis included 3 mice per group.

Murphy K.P., Hendley M.A., Patterson A.T., Hall H.E., Carter G.J., Isely C, & Gower R.M. (2020). Modulation of Adipocyte Size and Fat Pad Weight via Resveratrol Releasing Scaffolds Implanted into the Epididymal Adipose Tissue. Journal of biomedical materials research. Part A, 109(5), 766-778.

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