QCT NPs were synthesized by oxidative self-polymerization of QCT in the presence of NaIO4. In a typical procedure, QCT (10 mg, 0.033 mmol) was dissolved in 1 mL DMSO containing various amounts of NaIO4 corresponding to 1–4 molar excess of QCT. The solution was vigorously stirred at room temperature (RT) overnight. The next day, the DMSO solution was transferred to a dialysis membrane with 3.5 kD molecular weight cutoff (MWCO; Spectrum Laboratories Inc., Rancho Dominguez, CA, USA) and dialyzed against deionized water (4 L) for one day, changing the water every hour for the first 12 h. NPs were later centrifuged at 4,000× g for 5 min (Hermle Z230A centrifuge, Wehingen, Germany) to remove large aggregates. NPs were either stored as an aqueous dispersion at RT or lyophilized using a FreeZone 4.5 L Benchtop Freeze Dryer (Labconco Corporation, Kansas City, MO, USA).
Freezone 4.5 l benchtop freeze dryer
Manufactured by Labconco
Sourced in United States
The FreeZone 4.5 L Benchtop Freeze Dryer is a laboratory equipment designed for freeze-drying applications. It features a 4.5-liter drying chamber and can accommodate up to three 8-inch diameter shelves. The system operates at a temperature range of -50°C to +50°C and can achieve a vacuum pressure of 0.012 mbar. The FreeZone 4.5 L Benchtop Freeze Dryer is suitable for a variety of freeze-drying processes.
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3 protocols using freezone 4.5 l benchtop freeze dryer
1
Synthesis and Characterization of Quercetin Nanoparticles
2
FT-IR Characterization of TQ and mPEG-PCL
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FT-IR spectra were obtained using an IR Affinity-1 spectrometer (Shimadzu, Kyoto, Japan). KBr discs were prepared with powdered samples of TQ and mPEG-PCL, or TQ NPs which were lyophilized using a FreeZone 4.5 L Benchtop freeze dryer (Labconco Corporation, Kansas City, MO, USA).
3
Preparation of Coffee Extracts via Oxidation
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For the
preparation of air-oxidized extracts, ground coffee beans (2 g) were
weighed in a 100 mL Erlenmeyer flask, followed by adding boiling water
(25 mL), and the mixture was stirred vigorously for 15 min. Afterward,
the mixture was filtered using a filter paper to remove insoluble
coffee grounds and cooled to room temperature. The mixture was further
purified by UF (Pierce Protein Concentrator, 10 kD MW cutoff, Thermo
Scientific, Waltham, MA, USA) at 4000g and 4 °C
for 15 min (Hermle Z326K centrifuge, Wehingen, Germany), with repeated
washing with ultrapure water twice. For the preparation of NaIO4- and CuSO4-oxidized extracts, 200 mg of NaIO4 or CuSO4 was added to the ground beans (2 g) before
adding boiling water (25 mL), and the mixture was further processed
as described above. The obtained extracts were stored at 4 °C
or lyophilized using a FreeZone 4.5 L Benchtop Freeze Dryer (Labconco
Corporation, Kansas City, MO, USA).
preparation of air-oxidized extracts, ground coffee beans (2 g) were
weighed in a 100 mL Erlenmeyer flask, followed by adding boiling water
(25 mL), and the mixture was stirred vigorously for 15 min. Afterward,
the mixture was filtered using a filter paper to remove insoluble
coffee grounds and cooled to room temperature. The mixture was further
purified by UF (Pierce Protein Concentrator, 10 kD MW cutoff, Thermo
Scientific, Waltham, MA, USA) at 4000g and 4 °C
for 15 min (Hermle Z326K centrifuge, Wehingen, Germany), with repeated
washing with ultrapure water twice. For the preparation of NaIO4- and CuSO4-oxidized extracts, 200 mg of NaIO4 or CuSO4 was added to the ground beans (2 g) before
adding boiling water (25 mL), and the mixture was further processed
as described above. The obtained extracts were stored at 4 °C
or lyophilized using a FreeZone 4.5 L Benchtop Freeze Dryer (Labconco
Corporation, Kansas City, MO, USA).
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