Hcd2 cre mice

C57BL/6 wildtype mice were purchased from the Jackson Laboratory (Bar Harbor, ME). hCD2-Cre mice (Jackson Laboratory) were crossed with Raptor^fl/fl mice and Rictor^fl/fl mice (Jackson Laboratory)²⁸ (link) to generate CD2-cre; Raptor^fl/fl and CD2-cre; Rictor^fl/fl conditional knockout (cKO) mice. c-Maf^fl/fl mice⁶⁵ (link) were bred with Rorc-cre mice (Jackson Laboratory) to generate control c-Maf^fl/fl mice and Rorc-cre; c-Maf^fl/fl cKO mice. Male and female mice were used in all experiments (embryo and infant: E16-D1, adult: 6–12 weeks old) and were housed in specific pathogen free conditions. All animals were housed and treated in accordance with institutional guidelines and approved by the Institutional Animal Care and Use Committee at the University of Louisville, Louisville, KY.

C57BL/6 WT, MyD88^−/− and Il1r1^−/− mice on C57BL/6 background were purchased from the Jackson Laboratory. IL-23R KO mice were imported from Genetech. hCD2-Cre mice (Jackson Laboratory) were crossed with Raptor^fl/fl mice, Rictor^fl/fl mice (Jackson Laboratory) and Stat3^fl/fl mice (Ding et al., 2016 (link)) to generate CD2-cre;Raptor^fl/fl, CD2-cre;Rictor^fl/fl and CD2-cre;Stat3^fl/fl conditional KO (cKO) mice. Male and female mice were used in all experiments (6–12 weeks old) and were housed in specific pathogen free conditions. All experiments were in accordance with institutional guidelines and approved by the IACUC at the University of Louisville.