Mtser1 medium

Human embryonic stem cells (hESC, BR1 cell line) (Fraga et al., 2011 (link)) and iPS cells were cultured in mTSeR1 medium (Stemcell Technologies) on Matrigel-coated surface (BD Biosciences). The colonies were manually passaged every seven days and maintained at 37 °C in humidified air with 5% CO₂.

The following reagents were used for this study: mTSER1 medium, accutase, and Y-27632 (Stem Cell Technology, Seattle, WA, USA); Advanced RPMI 1640 medium (Gibco, Waltham, MA, USA); CHIR 99021 (Cayman, Ann Arbor, MI, USA); Activin A and FGF9 (Peprotech, Cranbury, NJ, USA); LPS (Sigma-Aldrich, Tokyo, Japan); Methylprednisolone (Pfizer, New York, NY, USA), Anti-GATA3 antibody and anti-cleaved Caspase-3 antibody (Cell Signaling Technology, Danvers, MA, USA); anti-HNF4α antibody, anti-synaptopodin antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-PAX2 antibody and anti-podocalyxin antibody (R&D Systems, Minneapolis, MN, USA), LTL fluorescence dye (Vector Laboratories, Burlingame, CA, USA); anti-E-cadherin antibody (BD Pharmingen, San Jose, CA, USA); and anti-TLR4 antibody and anti-Nrf2 antibody (Affinity Biosciences, Cincinnati, OH, USA).