Dnase

Manufactured by Vazyme

Sourced in China

DNase is a lab equipment product that degrades DNA. It functions by cleaving the phosphodiester bonds within the DNA molecule, effectively breaking down the DNA strands.

Automatically generated - may contain errors

Lab products found in correlation

Fv1000, by Olympus (3 mentions) Tunel brightgreen apoptosis detection kit, by Vazyme (3 mentions) Antifade mounting medium with dapi, by Beyotime (3 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Cfx96 real time pcr system, by Bio-Rad (1 mentions) Sosofast evagreen supermix, by Bio-Rad (1 mentions)

Close spelling variants of this product

DNase I (21 citations) RNase-free DNase I (5 citations) RNase-Free DNase Set (3 citations) RNase-Free DNase (2 citations) DNase and reverse transcriptase (2 citations)

4 protocols using dnase

RNA Extraction and RT-qPCR Analysis

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Following the manufacturer’s recommendations, TRIzol (Invitrogen) was used to extracted the total RNA from the pooled samples from the MGCs, CCs, and DOs, respectively. DNase (Vazyme Biotech, Nanjing, China) was applied to remove the residual genomic DNA before reverse transcription (RT). Approximately 0.5 μg of extracted RNA was used for each RT reaction. The complementary DNA was stored at −20 °C until use. RT-qPCR reactions were performed using a Bio-Rad CFX96 Real-Time PCR System with SosoFast EvaGreen Supermix (Bio-Rad Laboratories). The amplification protocol included an initial denaturation process at 95 °C for 30 s, followed by 40 cycles consisting of 5 s of denaturation at 95 °C and 5 s of annealing/extension at 60 °C. The negative controls were reactions without RT or the substitution of RNA samples with DEPC water (to detect any DNA contamination). The relative fold change of the mRNA transcripts was analyzed using the 2^−ΔΔCt method, with RPL19 or GAPDH mRNA serving as reference genes. The primers are listed in Table 1. Each experiment was performed in triplicate.

Lu J., Guo M., Wang X., Wang R., Xi G., An L., Tian J, & Chu M. (2023). A Redesigned Method for CNP-Synchronized In Vitro Maturation Inhibits Oxidative Stress and Apoptosis in Cumulus-Oocyte Complexes and Improves the Developmental Potential of Porcine Oocytes. Genes, 14(10), 1885.

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Apoptosis Analysis of Ishikawa Cells

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Ishikawa cells were grown on 14 mm glass coverslips at a density of 2 × 10 5 cells per well in a 24-well plate. 10 ng/mL TNF-α and 25 µg/mL ETA were used to treat the cell line. After 48 h of treatment, the cells were analyzed with the TUNEL BrightGreen Apoptosis Detection Kit (Vazyme, Inc., Nanjing, China), followed by mounting with antifade mounting medium with DAPI (Beyotime, Inc.) The analysis was conducted using a confocal laser scanning microscope (Olympus FV1000). The DNase (Vazyme, Inc., Nanjing, China) treated control group cells served as positive control. Each test had two replicates (n = 3).

Lv J., Shan X., Yang H., Wen Y., Zhang X., Chen H., Li H., Tian D., Wang C.C., Zhang R., Li T.C., Zhang X., Zhao X., Lu Y., Qin L., Zhu M, & Xu W. (2022). Single Cell Proteomics Profiling Reveals That Embryo-Secreted TNF-α Plays a Critical Role During Embryo Implantation to the Endometrium. Reproductive sciences (Thousand Oaks, Calif.), 29(5).

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Apoptosis Analysis of Ishikawa Cells

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Lv J., Shan X., Yang H., Wen Y., Zhang X., Chen H., Li H., Tian D., Wang C.C., Zhang R., Li T.C., Zhang X., Zhao X., Lü Y., Qin L., Zhu M., & Xu W. (2020). Single cell proteomics profiling reveals that embryo-secreted TNF-α plays a critical role during embryo implantation to the endometrium.

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Apoptosis Analysis in Ishikawa Cells

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Ishikawa cells were grown on 14 mm glass coverslips at a density of 2x10 5 cells per well of a 24-well plate. Then 10 ng/ml TNF-α and 25 μg/ml ETA were used to treat the cell line. After 48 hours treatment, cells were analysis with Tunel BrightGreen Apoptosis Detection Kit (Vazyme, Inc., Nanjing, China), followed by mounting with antifade mounting medium with DAPI (Beyotime, Inc., Shanghai, China). Then, analysis was conducted using a confocal laser scanning microscope (Olympus FV1000, Olympus Corporation). The DNase (Vazyme, Inc., Nanjing, China) treated control group cells served as positive control. Each test had two replicates (n=3).

Lv J., Shan X., Yang H., Wen Y., Xue-guang Z., Chen H., Li H., Dongmei T., Wang C.C., Zhang R., Chiu L.T., Xiao-hu Z., Xiaomiao Z., Lu Y., Qin L., Zhu M., & Wenming X. (2020). Single Cell Proteomics Profiling Revealing that Embryo Secreted TNF-α play Critical Role during Embryo Implantation to Endometrium.

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