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E cadherin decma 1

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E-cadherin (DECMA-1) is a cell adhesion molecule that plays a critical role in the formation and maintenance of adherens junctions between epithelial cells. It is a transmembrane glycoprotein that mediates calcium-dependent cell-cell adhesion.

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Lab products found in correlation

Axiovert 200m, by Zeiss (4 mentions) Antibody labeling kit, by Thermo Fisher Scientific (2 mentions) Active caspase 3, by BD (2 mentions) Axiovision 4, by Zeiss (2 mentions) Tissue tek o c t embedding compound, by Electron Microscopy Sciences (2 mentions) Vimentin sc 7557, by Santa Cruz Biotechnology (2 mentions) Neu sc 284, by Santa Cruz Biotechnology (2 mentions) Bio spin p30 columns, by Bio-Rad (2 mentions) Cd45 30 f11, by BioLegend (2 mentions) Horseradish peroxidase, by GE Healthcare (1 mentions) Bromodeoxyuridine (brdu), by Agilent Technologies (1 mentions) Goat anti trp2, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488 and 568, by Thermo Fisher Scientific (1 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) β catenin, by BD (1 mentions) Gapdh, by Merck Group (1 mentions) E cadherin, by BD (1 mentions)

3 protocols using e cadherin decma 1

1

Comprehensive Histological Profiling of Tumor Metastases

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The harvested primary tumors and PBS-perfused lungs bearing metastases were fixed in 4% paraformaldehyde overnight, followed by 30% sucrose for 2 days, and then embedded in Tissue-tek O.C.T. embedding compound (Electron Microscopy Sciences). Serial sections (10µm, at least 10 sections) were prepared for histological analysis by Hematoxylin & Eosin (H&E) staining, and immunofluorescent staining following standardized protocols.
Primary antibodies used in this study include CD45 (30-F11, BioLegend), E-cadherin (DECMA-1, BioLegend), vimentin (sc-7557, Santa Cruz), PyMT (ab15085, Abcam), Neu (sc-284, Santa Cruz), Ki67 (ab15580, Abcam), and Active Caspase-3 (C92–605, BD Pharmingen). Primary antibodies were directly conjugated to Alexa Fluor 647 using an antibody labeling kit (Invitrogen) performed as per manufacturer’s instructions and purified over BioSpin P30 columns (Bio-Rad). GFP+ and RFP+ cells were detected by inherent fluorescence.
Fluorescent images were obtained using a computerized Zeiss fluorescent microscope (Axiovert 200M), fitted with an apotome and an HRM camera. Images were analyzed using Axiovision 4.6 software (Carl Zeiss Inc.).
Fischer K.R., Durrans A., Lee S., Sheng J., Li F., Wong S., Choi H., El Rayes T., Ryu S., Troeger J., Schwabe R.F., Vahdat L.T., Altorki N.K., Mittal V, & Gao D. (2015). EMT is not required for lung metastasis but contributes to chemoresistance. Nature, 527(7579), 472-476.
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2

Antibody Panel for Cell Signaling Analysis

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The following antibodies were used in this study: rabbit antibodies directed against Par3 (no. 07-330; EMD Millipore), cleaved caspase3 (no. 9661; Cell Signaling Technology), phospho-Akt (Ser 473; 4060; Cell Signaling Technology), phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204; no. 4370; Cell Signaling Technology), rat anti–P-cadherin (PCD-1; no. 13-2000Z; hybridoma supernatant; Invitrogen; Nose and Takeichi, 1986 (link)), E-cadherin/DECMA-1 (no. 147302; BioLegend; Vestweber and Kemler, 1985 (link)), mouse anti–P-cadherin (no. 610228; BD), GAPDH (no. MAB374; EMD Millipore), α-tubulin (no. T-5168; clone B-5-1-2; Sigma-Aldrich), BrdU (no. MO744; Dako), E-cadherin (no. 610181; BD), β-catenin (no. 610154; BD), PCNA (no. NA03; clone PC10; EMD Millipore), and goat anti-TRP2 (no. sc-10451; Santa Cruz Biotechnology, Inc.). For secondary detection, Alexa Fluor 488 and 568 (Molecular Probes) or horse radish peroxidase (GE Healthcare) conjugates were used.
Mescher M., Jeong P., Knapp S.K., Rübsam M., Saynisch M., Kranen M., Landsberg J., Schlaak M., Mauch C., Tüting T., Niessen C.M, & Iden S. (2017). The epidermal polarity protein Par3 is a non–cell autonomous suppressor of malignant melanoma. The Journal of Experimental Medicine, 214(2), 339-358.
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3

Comprehensive Histological Profiling of Tumor Metastases

Check if the same lab product or an alternative is used in the 5 most similar protocols
The harvested primary tumors and PBS-perfused lungs bearing metastases were fixed in 4% paraformaldehyde overnight, followed by 30% sucrose for 2 days, and then embedded in Tissue-tek O.C.T. embedding compound (Electron Microscopy Sciences). Serial sections (10µm, at least 10 sections) were prepared for histological analysis by Hematoxylin & Eosin (H&E) staining, and immunofluorescent staining following standardized protocols.
Primary antibodies used in this study include CD45 (30-F11, BioLegend), E-cadherin (DECMA-1, BioLegend), vimentin (sc-7557, Santa Cruz), PyMT (ab15085, Abcam), Neu (sc-284, Santa Cruz), Ki67 (ab15580, Abcam), and Active Caspase-3 (C92–605, BD Pharmingen). Primary antibodies were directly conjugated to Alexa Fluor 647 using an antibody labeling kit (Invitrogen) performed as per manufacturer’s instructions and purified over BioSpin P30 columns (Bio-Rad). GFP+ and RFP+ cells were detected by inherent fluorescence.
Fluorescent images were obtained using a computerized Zeiss fluorescent microscope (Axiovert 200M), fitted with an apotome and an HRM camera. Images were analyzed using Axiovision 4.6 software (Carl Zeiss Inc.).
Fischer K.R., Durrans A., Lee S., Sheng J., Li F., Wong S., Choi H., El Rayes T., Ryu S., Troeger J., Schwabe R.F., Vahdat L.T., Altorki N.K., Mittal V, & Gao D. (2015). EMT is not required for lung metastasis but contributes to chemoresistance. Nature, 527(7579), 472-476.
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