Orius sc600 ccd camera

NP size and surface charge (Z potential) were determined by dynamic light scattering (DLS) at room temperature (Zetasizer Nano-ZSP, Malvern Instruments, UK). An aliquot (50 μL) of the NP suspension, obtained before lyophilization or after resuspending the lyophilized NPs in 1xPBS buffer, was added to 1 mL HPLC water. NP morphology was assessed utilizing transmission electron microscopy (TEM, JEM-1400 Plus, JEOL Ltd., Tokyo, Japan). Briefly, 5 μL samples were stained with NanoVan™ (Nanoprobes, NY, USA) and then placed on formvar/carbon-coated copper 200 mesh grids (EMS), and mixed with 5 μL of NV for 5–10 s. Excess stain was blotted off and grids were dried. The grids were viewed with Jeol® JEM-1400 Plus TEM (Jeol®, Tokyo, Japan). The grids were equipped with an ORIUS SC600 CCD camera (Gatan®, Abingdon, United Kingdom); the Gatan Microscopy Suite program was used (DigitalMicrograph, Gatan®, UK).

Transmission electron microscopy (TEM) was performed using a JEOL 1010 TEM (2001) at an accelerating voltage of 100 kV equipped with Gatan Orius SC600 CCD Camera (2014). X-ray powder diffraction (XRD) patterns were obtained on a Bruker AXS D4 Endeavor wide-angle XRD instrument with a Cu Kα radiation source at λ = 1.54056 Å. Upconversion emission spectra were analyzed using an Andor Shamrock SR-500i imaging spectrometer equipped with an iXon EMCCD Camera using a CW laser at the wavelength of 980 nm (Thorlabs, BL976-PAG900) as a near-infrared light source at a power of 1 mW. UV-visible absorption spectra were collected by using an Agilent Cary 60 UV-Vis Spectrophotometer. The morphology analysis of the samples was carried out with an FEI Verios 460L field emission gun scanning electron microscopy at an accelerating voltage of 3 kV. Atomic force microscopy imaging was conducted with a Bruker Dimension Icon. An 80-MHz Ti:sapphire femtosecond laser (Coherent, Chameleon Ultra II) tuned at the wavelength of 880 nm was used as the excitation source for TPE fluorescence imaging of the nanocomposite. A mercury light source (Olympus, U-LH100HG) was used to photochemically reduce the GO in water solution. Unless otherwise noted, characterizations of the synthesized materials were performed at room temperature.

Tetracycline-induced (TbUMSBP knockdown) and uninduced cells, were fixed in a solution containing 2% paraformaldehyde, 2.5 % glutaraldehyde EM grade, in 0.1 M sodium cacodylate buffer pH 7.3 for 2 h at room temperature followed by 24 h at 4°C. Cells were washed 4 times with sodium cacodylate and post fixed for 1 h in 1% osmium tetroxide, 1.5% potassium ferricyanide in sodium cacodylate, and washed 4 times with the same buffer, followed by dehydration with graded series of ethanol solutions (30, 50, 70, 80, 90, 95 %) for 10 min each and then 3 times with 100% ethanol for 20 min each, followed by 2 changes of propylene oxide. Cells were then infiltrated with a series of epoxy resins, (25, 50, 75, 100%) for 24 h each and polymerized in the oven at 60°C for 48 h. The blocks were sectioned by an ultramicrotome, Ultracut E, Riechert-Jung, and sections of 80 nm were obtained and stained with uranyl acetate and lead citrate. Sections were observed by Jeol® JEM-1400 Plus TEM (Jeol®, Tokyo, Japan), equipped with ORIUS SC600 CCD camera (Gatan®, Abingdon, United Kingdom), and Gatan Microscopy Suite program (DigitalMicrograph, Gatan®, UK).