Sodium phosphate dibasic anhydrous salt

Nebivolol hydrochloride (1-(6-fluoro-3,4-dihydro-2H-chromen-2-yl)-2-[[2-(6-fluoro-3,4-dihydro-2H-chromen-2-yl)-2-hydroxyethyl]amino]ethanolhydrochloride) was obtained from commercially available pharmaceutical formulation—Nebilet 5 mg tablets (Berlin-Chemie Menarini, Glienicker Weg, Germany) containing racemic mixture of SRRR-nebivolol and RSSS-nebivolol (1:1). Twenty tablets (equivalent of 40.5 mg of nebivolol) were grounded in mortar, then weighted and swept for 5 min with 25 mL of methanol, and then diluted with water to the working concentration.
Water (LC-MS grade), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), HLM, sodium phosphate monobasic monohydrate salt and sodium phosphate dibasic anhydrous salt were obtained from Sigma-Aldrich (St. Louis, CA, USA). Acetonitrile (LC-MS grade) was purchased from Merck (Darmstadt, Germany) and 98% formic acid (MS grade) was obtained from Fluka (Taufkirchen, Germany).

Dapoxetine hydrochloride ((S)-N,N-dimethyl-1-phenyl-3-(1-naphthalenyloxy)propanamine hydrochloride, purity ≥ 98%), water (LC–MS grade), β-nicotinamide adenine dinucleotide 2′-phosphate-reduced tetrasodium salt hydrate (NADPH), HLM, sodium phosphate monobasic monohydrate salt and sodium phosphate dibasic anhydrous salt were obtained from Sigma-Aldrich (St. Louis, CA, USA). Acetonitrile (hypergrade for LC–MS) was purchased from Merck (Darmstadt, Germany) and 98% formic acid (MS grade) was obtained from Fluka (Taufkirchen, Germany).