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Ld assay kit

Manufactured by Nanjing Jiancheng
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Sourced in China

The LD assay kit is a laboratory diagnostic tool used to quantify the levels of lactate dehydrogenase (LD) in biological samples. LD is an enzyme found in various tissues and is often used as a biomarker for certain medical conditions. The kit provides reagents and protocols to measure LD activity colorimetrically or spectrophotometrically, allowing for the assessment of LD levels in the sample.

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Lab products found in correlation

Nefa assay kit, by Nanjing Jiancheng (1 mentions) Mouse insulin elisa kit, by Cusabio (1 mentions) Mouse il 13 elisa kit, by MultiSciences Biotech (1 mentions) Ldh activity assay kit, by Nanjing Jiancheng (1 mentions)

2 protocols using ld assay kit

1

Serum Biomarker Quantification Protocol

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Serum TG and cholesterol were measured using the Chemistry Analyzer (Rayto Chemray800). Serum lactate, FFA, glycerol, insulin, and IL-13 were measured using the LD assay kit (A019-2; Nanjing Jiancheng), NEFA assay kit (A042-2-1; Nanjing Jiancheng), glycerol content assay kit (F005-1-1; Nanjing Jiancheng), mouse insulin ELISA Kit (CSB-E05071m; CUSABIO), and mouse IL-13 ELISA Kit (EK213/2; Multi Sciences), respectively.
Rong Y., Zhang S., Nandi N., Wu Z., Li L., Liu Y., Wei Y., Zhao Y., Yuan W., Zhou C., Xiao G., Levine B., Yan N., Mou S., Deng L., Tang Z., Liu X., Kramer H, & Zhong Q. (2022). STING controls energy stress-induced autophagy and energy metabolism via STX17. The Journal of Cell Biology, 221(7), e202202060.
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2

Tissue Homogenization and Enzyme Analysis

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The tissues were homogenized on ice as a 1:9 (w/v) dilution in 0.9% physiological saline. The homogenate was centrifuged at 2500 rpm/min at 4 °C for 10 min, and the supernatant was collected. Protein level was measured by Bradford assay. The LD assay kit and the LDH activity assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing China) were used to assess the LD content and LDH activity in tissues.
Wei D., Wei L., Li X., Wang Y, & Wei L. (2016). Effect of Hypoxia on Ldh-c Expression in Somatic Cells of Plateau Pika. International Journal of Environmental Research and Public Health, 13(8), 773.
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